Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	75,708	G→A	K48K (AAG→AAA)	SAUSA300_RS00340 →	universal stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	75,708	0	G	A	100.0%	60.9 / NA	22	K48K (AAG→AAA)	SAUSA300_RS00340	universal stress protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/3);  total (19/3)

CCCTGACTATTTAGAAAAAGTCACCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAGTATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAGAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGATA  >  NC_007793/75618‑75793                                                                                           |                                                                                      accTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTataat                                                                              >  1:2907621/2‑100 (MQ=255)     gACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGc                                                                          >  1:2924965/1‑100 (MQ=255)         aTTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATaa                                                                      <  1:579193/100‑1 (MQ=255)         aTTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATaa                                                                      >  1:5725863/1‑100 (MQ=255)                           aTGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTagaga                                                    <  1:1140261/100‑1 (MQ=255)                                           tgtAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAactc                                    >  1:1102487/1‑100 (MQ=255)                                                           gTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTg                    >  1:4203641/1‑100 (MQ=255)                                                             aaCAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAAAAAAAAAAATCAATCCCTTTTTaacggaa                  >  1:4676626/1‑94 (MQ=255)                                                                        aTCTTGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAag       <  1:2754868/100‑1 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTATAAAAAAGAGat   >  1:3021950/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:596209/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:4780665/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:4707936/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:4467156/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:433408/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:4244415/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:3761745/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:3673030/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:3251474/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGat   >  1:2681230/1‑100 (MQ=255)                                                                            tGCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGA‑AAAAAACTCACTCCATTTTTACGTGAATTAGAGAAAAGAGatc  >  1:346909/1‑99 (MQ=255)                                                                             gCAATTAGTGTCAAATATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAAAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGata  >  1:3142098/1‑100 (MQ=255)                                                                                           |                                                                                      CCCTGACTATTTAGAAAAAGTCACCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAGTATTATAATAAGCATAAAGAAGATATTATTAGAGAAAAAGAGAAAAAACTCACTCCATTTTTACGTGAATTAGAAAAAAGAGATA  >  NC_007793/75618‑75793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑