Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	75,641	C→T	T26I (ACC→ATC)	SAUSA300_RS00340 →	universal stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	75,641	0	C	T	100.0%	58.2 / NA	21	T26I (ACC→ATC)	SAUSA300_RS00340	universal stress protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/2);  total (19/2)

TAATAGGAGGCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCACCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAGTATTATAATAAGCATAA  >  NC_007793/75546‑75725                                                                                                |                                                                                     tgctAGGAGGCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGAt                                                                                  >  1:1590738/4‑100 (MQ=255)  gcagggaggCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGTTg                                                                                 >  1:5191411/5‑100 (MQ=255)   aTAGGAGGCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGa                                                                                <  1:1427832/100‑1 (MQ=255)    caggaggCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGaa                                                                               >  1:2271997/2‑100 (MQ=255)    caggaggCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTACCCCTGACTATTTAGAAAAAGTCATCGATGaa                                                                               >  1:236889/2‑100 (MQ=255)          gCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCa                                                                         >  1:233561/1‑100 (MQ=255)          gCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGACGAAGAtcgg                                                                         >  1:3094921/1‑97 (MQ=255)             ggTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAgtt                                                                      >  1:79749/1‑100 (MQ=255)             ggTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAgtt                                                                      >  1:31241/1‑100 (MQ=255)             ggTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAgtt                                                                      >  1:1677795/1‑100 (MQ=255)                                  attattACCCTATGATTTCGAAAACGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTa                                                 >  1:3446862/1‑100 (MQ=255)                                     attaCCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACa                                              >  1:294323/1‑100 (MQ=255)                                       taCCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGa                                            >  1:2596627/1‑100 (MQ=255)                                        aCCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACaaaa                                           >  1:5562743/1‑100 (MQ=255)                                        aCCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGaa                                           >  1:2613382/1‑100 (MQ=255)                                        aCCCTATGATTTCGAAAATGATTTTAGTGCCATCCCTGACCATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGaa                                           >  1:2254482/1‑100 (MQ=255)                                             aTGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGa                                      >  1:3439367/1‑100 (MQ=255)                                                                         accTGACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTataat          >  1:2907621/2‑100 (MQ=255)                                                                             gACTATTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGc      >  1:2924965/1‑100 (MQ=255)                                                                                 aTTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATaa  >  1:5725863/1‑100 (MQ=255)                                                                                 aTTTAGAAAAAGTCATCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAATATTATAATAAGCATaa  <  1:579193/100‑1 (MQ=255)                                                                                                |                                                                                     TAATAGGAGGCTGGTGTAGATGTTCAAAAATATATTATTACCCTATGATTTCGAAAATGATTTTAGTGCTATCCCTGACTATTTAGAAAAAGTCACCGATGAAGATTCAGTTGTTGTAATTTATCACGTTGTAACAGAAAATGATCTTGCAATTAGTGTCAAGTATTATAATAAGCATAA  >  NC_007793/75546‑75725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑