Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	74,699	A→G	I122T (ATT→ACT)	SAUSA300_RS00335 ←	ArgR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	74,699	0	A	G	100.0%	16.8 / NA	8	I122T (ATT→ACT)	SAUSA300_RS00335	ArgR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

TTTAAGGGAATAATTGATAACAAACAAATTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAATTCCTAATATTTCTTTCATTTGTAATTGATCTATGTAATAACTTATACTTTGAGCAAAGCCAGG  >  NC_007793/74616‑74763                                                                                    |                                                                 tttAAGGGAATAATTGATAACAAACAAATTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATAtttcttt                                                  >  1:3367349/1‑100 (MQ=255)    aaGGGAATAATTGATAACAAACAAATTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCAt                                               >  1:3399897/1‑100 (MQ=255)                   aacaaacaAATTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTAt                                >  1:4001669/1‑100 (MQ=255)                      aaacaaaTTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTATGta                             >  1:1097394/1‑100 (MQ=255)                                          tttGAAGAAGTCAAAATCATCAAAGTATCGTTACCCCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTATGTAATAACTTATACTTTGAGCaa         <  1:1838068/100‑1 (MQ=255)                                              aagaagTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTATGTAATAACTTATACTTTGAGCAAAGcc     <  1:5004290/100‑1 (MQ=255)                                                 aagTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTGTGTAATAACTTATACTTTGAGCAAAGCCAgg  <  1:5512348/100‑1 (MQ=255)                                                 aagTCAAAATCATCAAAGTATCGTTACCTCCAATAGTTCCTAATATTTCTTTCATTTGTAATTGATCTATGTAATAACTTATACTTTGAGCAAAGCCAgg  <  1:2982528/100‑1 (MQ=255)                                                                                    |                                                                 TTTAAGGGAATAATTGATAACAAACAAATTCTGCTATTTCATTTGAAGAAGTCAAAATCATCAAAGTATCGTTACCTCCAATAATTCCTAATATTTCTTTCATTTGTAATTGATCTATGTAATAACTTATACTTTGAGCAAAGCCAGG  >  NC_007793/74616‑74763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑