Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	74,079	G→A	D90D (GAC→GAT)	SAUSA300_RS00330 ←	arginine deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	74,079	0	G	A	100.0%	49.5 / NA	18	D90D (GAC→GAT)	SAUSA300_RS00330	arginine deiminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/17);  total (1/17)

TTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATGTCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCTGCTGCAA  >  NC_007793/73986‑74131                                                                                              |                                                     tttatttaCAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAACATATCGttt                                                <  1:5547187/100‑1 (MQ=255)   tatttaCAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTag                                              <  1:2379431/100‑2 (MQ=255)               cTTGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTcgcg                                  <  1:4714585/100‑1 (MQ=255)                ttGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGt                                 <  1:5039785/100‑1 (MQ=255)                       gATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTg                          >  1:2466421/1‑100 (MQ=255)                        aTAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACtagtat                         <  1:744038/100‑6 (MQ=255)                           aCTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGccc                      <  1:4665180/100‑3 (MQ=255)                             tttGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAg                    <  1:4691338/100‑1 (MQ=255)                              ttGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGt                   <  1:473394/100‑1 (MQ=255)                               tGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTa                  <  1:1009193/100‑1 (MQ=255)                                 aaaaGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAAt                <  1:3578467/100‑1 (MQ=255)                                 aaaaGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAAt                <  1:5248391/100‑1 (MQ=255)                                 aaaaGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAAt                <  1:5794755/100‑1 (MQ=255)                                            ttAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTctgctg     <  1:1439487/100‑1 (MQ=255)                                             tAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCtgctgc    <  1:3147283/100‑1 (MQ=255)                                              aaTTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCTGCTGCa   <  1:5461740/100‑1 (MQ=255)                                              aaTTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCTGCTGCa   <  1:1897272/100‑1 (MQ=255)                                               aTTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATATCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCTGCTGCaa  <  1:4378862/100‑1 (MQ=255)                                                                                              |                                                     TTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAATTTCAGTTTCATGACCTAATATTGTCTTTTTAGATTCTGTTAATATGTCGTTTATGAAGTTCTCGCGTACTTCTGGCTCAGTAATAGATTCTGCTGCAA  >  NC_007793/73986‑74131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑