Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	73,941	G→A	T136T (ACC→ACT)	SAUSA300_RS00330 ←	arginine deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	73,941	0	G	A	98.9%	257.1 / ‑6.2	92	T136T (ACC→ACT)	SAUSA300_RS00330	arginine deiminase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (38/51);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTACCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGGGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAA  >  NC_007793/73845‑74032                                                                                                 |                                                                                            tctACCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgtt                                                                                          <  1:3569931/100‑1 (MQ=255)  ctACCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttg                                                                                         >  1:1968523/1‑100 (MQ=255)   tACCAATTGAAGCTTGGGGATCTCTTGTTAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgt                                                                                        >  1:2682479/1‑100 (MQ=255)    aCCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgtt                                                                                       >  1:4179548/1‑100 (MQ=255)    aCCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgtt                                                                                       >  1:3171715/1‑100 (MQ=255)    aCCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgtt                                                                                       >  1:5575648/1‑100 (MQ=255)    aCCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgtt                                                                                       >  1:336734/1‑100 (MQ=255)     ccAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAgttgtaa                                                                                      <  1:375278/100‑3 (MQ=255)      cAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTc                                                                                     <  1:2529899/100‑1 (MQ=255)       ccgatctgCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCa                                                                                    <  1:4669099/93‑1 (MQ=255)       aaTTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCa                                                                                    <  1:1259192/100‑1 (MQ=255)          tGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGt                                                                                 >  1:1541852/1‑100 (MQ=255)          tGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGt                                                                                 >  1:2114686/1‑100 (MQ=255)                gtGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAAtt                                                                           >  1:5258986/2‑100 (MQ=255)                 tGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAAttt                                                                          >  1:5137478/1‑100 (MQ=255)                   gggATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATttct                                                                        <  1:3124458/100‑1 (MQ=255)                          ctTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtac                                                                 >  1:810359/1‑100 (MQ=255)                          ctTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtac                                                                 >  1:2357006/1‑100 (MQ=255)                          ctTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtac                                                                 >  1:3883831/1‑100 (MQ=255)                          ctTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtac                                                                 >  1:4107626/1‑100 (MQ=255)                            tGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtacgt                                                               >  1:1095061/1‑100 (MQ=255)                            tGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtacgt                                                               >  1:4669245/1‑100 (MQ=255)                            tGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtacgt                                                               <  1:5676112/100‑1 (MQ=255)                             gTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtacgta                                                              <  1:2481153/100‑1 (MQ=255)                               aaaaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTtacgtcgg                                                            <  1:4353689/100‑4 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          <  1:4215970/100‑1 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          >  1:3389673/1‑100 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          <  1:4732440/100‑1 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          <  1:3301694/100‑1 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          <  1:1312704/100‑1 (MQ=255)                                 aaTAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACacc                                                          <  1:5418558/100‑1 (MQ=255)                                 aaTAAAAGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgcc                                                          <  1:4303017/100‑1 (MQ=255)                                   tAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccat                                                        <  1:2138735/100‑2 (MQ=255)                                   tAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccag                                                        <  1:3376459/100‑1 (MQ=255)                                   atgtggTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccag                                                        >  1:872853/5‑100 (MQ=255)                                    ctaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagc                                                       >  1:1213387/3‑100 (MQ=255)                                    caaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagc                                                       >  1:749259/2‑100 (MQ=255)                                    aaaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagg                                                       <  1:5501472/100‑2 (MQ=255)                                    aaaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagc                                                       >  1:2785626/1‑100 (MQ=255)                                    aaaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagc                                                       >  1:5013699/1‑100 (MQ=255)                                    aaaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccaga                                                       <  1:4594822/100‑2 (MQ=255)                                     aaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagcc                                                      <  1:367481/100‑1 (MQ=255)                                     aaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccactg                                                      <  1:2787763/100‑4 (MQ=255)                                     aaGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATGCTACTAAATGAGTTGTTTCAAGTTGAATTTCGTCTTTACGTACgccagag                                                      <  1:5189842/100‑3 (MQ=255)                                       ggTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCAt                                                    <  1:2712662/100‑1 (MQ=255)                                       ggTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCAt                                                    <  1:1303269/100‑1 (MQ=255)                                       ggTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCAt                                                    <  1:1742494/100‑1 (MQ=255)                                        tctGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagatcgg                                                   >  1:4121564/3‑95 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGGGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATg                                                   >  1:5231887/1‑100 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagccagg                                                   <  1:2462685/100‑3 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATg                                                   <  1:306519/100‑1 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATg                                                   <  1:29655/100‑1 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATg                                                   <  1:397483/100‑1 (MQ=255)                                        gTTGGGCATTGGATCTAAGTAAAAGGGATATCTCTCATCAAGAGAGGAGAAGAAATATTTTTTGTTAAGTTGAATTTCTTCTTTACGTACGCCAGCCAtt                                                   <  1:228457/100‑2 (MQ=255)                                        gTTGGGCATTGGACCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATg                                                   <  1:1656296/100‑1 (MQ=255)                                        cttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagatcgg                                                   >  1:5263167/2‑95 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATCTATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagccagat                                                  >  1:5586417/1‑97 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagccgaga                                                  >  1:1691966/1‑96 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagatcgga                                                  >  1:2310586/1‑94 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATgg                                                  <  1:536060/100‑2 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGa                                                  <  1:5579067/100‑1 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGa                                                  <  1:3172735/100‑1 (MQ=255)                                         ttGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGa                                                  <  1:1977300/100‑1 (MQ=255)                                          tGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACgccagccagatc                                                 >  1:2374905/1‑96 (MQ=255)                                          tGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAt                                                 >  1:2213203/1‑100 (MQ=255)                                          tGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAt                                                 >  1:5208380/1‑100 (MQ=255)                                          tGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAt                                                 >  1:2635325/1‑100 (MQ=255)                                           gggCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGCGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAtt                                                >  1:4141566/1‑100 (MQ=255)                                           gggCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAtt                                                >  1:4475750/1‑100 (MQ=255)                                            ggCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGAttt                                               <  1:1917695/100‑1 (MQ=255)                                             gCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGacacg                                              <  1:221085/100‑5 (MQ=255)                                                        aaGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGtt                                   >  1:4387168/1‑100 (MQ=255)                                                        aaGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGtt                                   >  1:5088955/1‑100 (MQ=255)                                                        aaGTAAAATGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGtt                                   >  1:1411782/1‑100 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTGCTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:2506649/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:3269794/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:132333/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:3075240/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:2718743/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:5043963/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:4776456/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:4338383/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:2179630/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:248266/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTc                            <  1:2447877/100‑1 (MQ=255)                                                               aTGGATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTCggcc                            <  1:3514137/100‑3 (MQ=255)                                                                 ggATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGTCAGCCATGATTTTATTTACAAGTTCTTGGTCAg                          <  1:4393595/100‑1 (MQ=255)                                                                 ggATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAg                          <  1:5214310/100‑1 (MQ=255)                                                                 ggATATCTATCATCCATATATTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAg                          <  1:1692070/100‑1 (MQ=255)                                                                             tcCATATATTCTACTAAATGAGTTGTTTCTAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACACGTTCTTGGTCAGATAACCTTGaaa              >  1:2370762/1‑100 (MQ=255)                                                                                   tatTCTACTAAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAAtt        >  1:2128649/1‑100 (MQ=255)                                                                                         actaAATGAGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTaa  >  1:682417/1‑100 (MQ=255)                                                                                                 |                                                                                            TCTACCAATTGAAGCTTGGGGATCTCTTGTAAAATAAAGGTTGGGCATTGGATCTAAGTAAAATGGATATCTATCATCCATATATTCTACTAAATGGGTTGTTTCAAGTTGAATTTCTTCTTTACGTACGCCAGCCATGATTTTATTTACAAGTTCTTGGTCAGATAACTTTGAAAAGAATTCTTTAA  >  NC_007793/73845‑74032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑