Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	72,335	C→T	K231K (AAG→AAA)	SAUSA300_RS00325 ←	amino acid APC transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	72,335	0	C	T	100.0%	30.4 / NA	12	K231K (AAG→AAA)	SAUSA300_RS00325	amino acid APC transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

GCTGAATGACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTA  >  NC_007793/72247‑72420                                                                                         |                                                                                      gctgAATGACACCTTGGGCTAGTACAGTCATTAAGAATTAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACtt                                                                            >  1:5571447/1‑100 (MQ=255)    gAATGACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTgg                                                                         <  1:472942/100‑2 (MQ=255)    gAATGACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGc                                                                         >  1:5705226/1‑100 (MQ=255)     aaTGACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCa                                                                        <  1:849159/100‑1 (MQ=255)        gACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGt                                                                     >  1:3373040/1‑100 (MQ=255)                                       taaatGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCtg                                      <  1:5518185/100‑1 (MQ=255)                                         aatGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCtgtg                                    <  1:3058567/100‑1 (MQ=255)                                          atGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGa                                   <  1:2826902/100‑1 (MQ=255)                                                aGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATAccc                             <  1:4096716/100‑1 (MQ=255)                                                           cAAACCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACt                  <  1:1652881/100‑1 (MQ=255)                                                            aaaCCAATAACGGTAGCAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTa                 <  1:1241844/100‑1 (MQ=255)                                                                           ccAGTTCCTACATCTTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTa  >  1:1279983/2‑100 (MQ=255)                                                                                         |                                                                                      GCTGAATGACACCTTGGGCTAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTA  >  NC_007793/72247‑72420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑