Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	70,190	A→G	D216D (GAT→GAC)	SAUSA300_RS00315 ←	ornithine carbamoyltransferase 1, catabolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	70,190	0	A	G	100.0%	63.6 / NA	26	D216D (GAT→GAC)	SAUSA300_RS00315	ornithine carbamoyltransferase 1, catabolic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/18);  total (8/18)

TCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTT  >  NC_007793/70107‑70273                                                                                    |                                                                                    agtgtgccTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGAttt                                                                     >  1:4913581/8‑100 (MQ=255)       atctcatcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTcaccag                                                               <  1:2413801/97‑2 (MQ=255)             tcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGtttt                                                         <  1:3947520/100‑1 (MQ=255)                 tcgcgcccATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:1405072/95‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:2372981/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:811865/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:2795150/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:705878/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:3706457/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:610805/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:5276381/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:4653808/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:4727428/100‑1 (MQ=255)                 gTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTc                                                     <  1:1338443/100‑1 (MQ=255)                   tCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAg                                                   <  1:589848/100‑1 (MQ=255)                    cACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc                                                  >  1:1927755/1‑100 (MQ=255)                    cACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc                                                  >  1:2114278/1‑100 (MQ=255)                    cACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc                                                  <  1:2574894/100‑1 (MQ=255)                      cccATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCta                                                >  1:2084555/1‑100 (MQ=255)                          tAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATc                                            <  1:4105682/100‑1 (MQ=255)                              tACCCAAACATCTGTGTATATTACATCAGATCCTTTGACACCTTCTTCACTATCGTCAGTTATAAGGATTTCACCACCGTTTTTTTCAGCTCTATCAttc                                        >  1:5596321/1‑99 (MQ=255)                                                  ttACATCAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATTTAATAATtcatc                    <  1:180789/100‑1 (MQ=255)                                                        cAGATCCTTTGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATTTAATAATTCATCAGTTgg              >  1:1684154/1‑100 (MQ=255)                                                                ttGACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATTTAATAATTCATCAGTTGGATTTAGtt      >  1:2858139/1‑100 (MQ=255)                                                                 ggACACCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATTTAATAATTCATCAGTTGGATTTAGTTc     >  1:4374427/2‑100 (MQ=255)                                                                    cacCTTCATCAATATCGTCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATTTAATAATTCATCAGTTGGATTTAGTTCttt  <  1:4799023/100‑1 (MQ=255)                                                                                    |                                                                                    TCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTT  >  NC_007793/70107‑70273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑