Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	68,479	G→A	G172S (GGT→AGT)	SAUSA300_RS00305 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	68,479	0	G	A	100.0%	26.7 / NA	12	G172S (GGT→AGT)	SAUSA300_RS00305	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

AATTGTGGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGGGTTGTATATTACATAGTGATCAAGGCAGCGTTTATACATCTTATGCTTATTATCAATTGTGCGAAGAAA  >  NC_007793/68400‑68548                                                                                |                                                                      caTTGTGGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGAt                                                   >  1:3491867/2‑100 (MQ=25)   ttGTGGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAAGGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCa                                                 >  1:4661280/1‑100 (MQ=255)   ctgtgGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCa                                                 <  1:5114162/99‑1 (MQ=255)     gtgGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAg                                               >  1:3104611/1‑100 (MQ=25)     gtgGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAg                                               >  1:4034833/1‑100 (MQ=25)     gtgGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAg                                               >  1:5431648/1‑100 (MQ=25)       ggCGTATAAAATAGATGATAGACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAGGc                                             <  1:4047102/100‑1 (MQ=255)       ggCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGtagatcggaa                                             >  1:2374804/1‑91 (MQ=11)         cGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTGTATTACATAGTGATCAAGGCAg                                           <  1:2431992/100‑1 (MQ=255)          gTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAGGCAGc                                          <  1:1482066/100‑1 (MQ=25)                                            gTTAATGATACATTAAATCAAATCGATATACCTGAGAGTTGTATATTACATAGTGATCAAGGCAGCGTTTATACATCTTATGCTTATTATCAATTGTGCg        <  1:4754006/100‑1 (MQ=25)                                                  aaTACATTAAATCAAATCGATGTACCTGAGAGTTGTATATTACATAGTGATCAAGGCAGCGTTTATACATCTTATGCTTATTATCAATTGTGCGAAGaaa  >  1:4215989/2‑100 (MQ=255)                                                                                |                                                                      AATTGTGGCGTATAAAATAGATGATAAACAAGACCAAAGTTTAGTTAATGATACATTAAATCAAATCGATATACCTGAGGGTTGTATATTACATAGTGATCAAGGCAGCGTTTATACATCTTATGCTTATTATCAATTGTGCGAAGAAA  >  NC_007793/68400‑68548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑