Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,637	G→A	intergenic (‑49/+463)	SAUSA300_RS00270 ← / ← SAUSA300_RS00275	transposase/spermidine N1‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,637	0	G	A	100.0%	21.8 / NA	9	intergenic (‑49/+463)	SAUSA300_RS00270/SAUSA300_RS00275	transposase/spermidine N1‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/8);  total (1/8)

ACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTCAGTCTAAAATTAAA  >  NC_007793/62545‑62681                                                                                             |                                              aCTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGttttt                                        >  1:1642627/1‑100 (MQ=255)            cTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTccc                             <  1:4722789/100‑1 (MQ=255)               tGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACt                          <  1:320370/100‑1 (MQ=255)                         gAATAAGAAACTCTTTTCATAGAAAAAAACGCCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAACTGGGGTc                <  1:3156216/100‑1 (MQ=255)                         gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc                <  1:1391135/100‑1 (MQ=255)                         gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc                <  1:3995734/100‑1 (MQ=255)                                   ctctTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacata      <  1:3907763/100‑5 (MQ=255)                                   ctctTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacacc      <  1:3553785/100‑5 (MQ=255)                                       tttCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacaggccac  <  1:4450438/100‑9 (MQ=255)                                                                                             |                                              ACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTCAGTCTAAAATTAAA  >  NC_007793/62545‑62681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑