Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,578	G→A	A4V (GCT→GTT)	SAUSA300_RS00270 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,578	0	G	A	100.0%	14.6 / NA	7	A4V (GCT→GTT)	SAUSA300_RS00270	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

TGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTC  >  NC_007793/62517‑62667                                                              |                                                                                           ttttgAAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCAtt                                                      >  1:4436596/3‑100 (MQ=255)                             aCTGCTTTATACTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGttttt                          >  1:1642627/1‑100 (MQ=255)                                        cTTTGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTccc               <  1:4722789/100‑1 (MQ=255)                                           tGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACt            <  1:320370/100‑1 (MQ=255)                                                     gAATAAGAAACTCTTTTCATAGAAAAAAACGCCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAACTGGGGTc  <  1:3156216/100‑1 (MQ=255)                                                     gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc  <  1:1391135/100‑1 (MQ=255)                                                     gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc  <  1:3995734/100‑1 (MQ=255)                                                              |                                                                                           TGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATACTTTGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTC  >  NC_007793/62517‑62667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑