Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,518	G→T	T24K (ACA→AAA)	SAUSA300_RS00270 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,518	0	G	T	100.0%	33.2 / NA	13	T24K (ACA→AAA)	SAUSA300_RS00270	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/9);  total (4/9)

TAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATA  >  NC_007793/62434‑62555                                                                                     |                                      ccgatctCCCCACTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCctgctg                        <  1:5706808/94‑1 (MQ=255)   tcTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct                      <  1:3382139/99‑1 (MQ=255)   tcTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct                      <  1:5686312/99‑1 (MQ=255)   gatctCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct                      <  1:4383812/96‑1 (MQ=255)    cTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgcta                     <  1:745130/100‑2 (MQ=255)    cTTTCCCCATTTCGATAACATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgctg                     <  1:4560042/100‑2 (MQ=255)     tttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCttt                    <  1:4151114/100‑1 (MQ=255)      ttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCtttc                   >  1:5443018/1‑100 (MQ=255)      ttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCCTTTTTTGAAAATCCTGCTGCtttc                   >  1:5527977/1‑100 (MQ=255)       tCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCtttca                  >  1:3053264/1‑100 (MQ=255)                 gATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTgg        <  1:1366119/100‑2 (MQ=255)                      cATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTGCTTtag   <  1:1819990/100‑2 (MQ=255)                       aTCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTGCTTtata  >  1:2678912/1‑100 (MQ=255)                                                                                     |                                      TAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATA  >  NC_007793/62434‑62555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑