| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 62,467 | T→G | N41T (AAT→ACT) | SAUSA300_RS00270 ← | transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 62,467 | 0 | T | G | 93.8% | 33.0 / ‑4.2 | 16 | N41T (AAT→ACT) | SAUSA300_RS00270 | transposase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (5/10); minor base A (0/1); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTGAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATA > NC_007793/62372‑62555 | tCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAgtttt < 1:976821/100‑1 (MQ=255) cAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAATTTTc < 1:3646151/100‑1 (MQ=255) tctgccGTTGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTa < 1:2797804/96‑1 (MQ=38) cAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTc > 1:5192952/1‑100 (MQ=255) ccgatctCCCCACTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCctgctg < 1:5706808/94‑1 (MQ=255) tcTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct < 1:5686312/99‑1 (MQ=255) tcTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct < 1:3382139/99‑1 (MQ=255) gatctCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgct < 1:4383812/96‑1 (MQ=255) cTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgcta < 1:745130/100‑2 (MQ=255) cTTTCCCCATTTCGATAACATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTgctgctg < 1:4560042/100‑2 (MQ=255) tttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCttt < 1:4151114/100‑1 (MQ=255) ttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCtttc > 1:5443018/1‑100 (MQ=255) ttCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCCTTTTTTGAAAATCCTGCTGCtttc > 1:5527977/1‑100 (MQ=255) tCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCtttca > 1:3053264/1‑100 (MQ=255) gATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTgg < 1:1366119/100‑2 (MQ=255) cATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTGCTTtag < 1:1819990/100‑2 (MQ=255) aTCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTTTTGAAAATCCTGCTGCTTTCATTTCAACTGCTTtata > 1:2678912/1‑100 (MQ=255) | TCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTGAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTCTTTTGTTGGAAATCCTGCTGCTTTCATTTCAACTGCTTTATA > NC_007793/62372‑62555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |