Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,425	G→A	S55L (TCA→TTA)	SAUSA300_RS00270 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,425	0	G	A	100.0%	45.8 / NA	17	S55L (TCA→TTA)	SAUSA300_RS00270	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/15);  total (2/15)

GTACTCTCTTATGATTAATTGGTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTGAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTC  >  NC_007793/62330‑62513                                                                                                |                                                                                         gTACTCTCTTATGATTAATTGGTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAaaaa                                                                                      >  1:2874090/1‑100 (MQ=255)           aTGATTAATTGGTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACtt                                                                            <  1:4869080/100‑1 (MQ=255)                 aaTTGGTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGGTTACCAACATGTTGTAAAAATCTATAACTTTCCCCa                                                                      <  1:4603309/100‑1 (MQ=255)                     ggTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCAttag                                                                  <  1:1497035/100‑3 (MQ=255)                           gTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATAcc                                                            <  1:556422/100‑1 (MQ=255)                            tataACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCa                                                           <  1:5435526/100‑1 (MQ=255)                            tataACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCa                                                           <  1:3963126/100‑1 (MQ=255)                             ataACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCac                                                          <  1:3620837/100‑2 (MQ=255)                              taACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCAtg                                                         <  1:3717535/100‑2 (MQ=255)                                aCATTGATTAGTCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCaggta                                                       <  1:527651/100‑5 (MQ=255)                                     gATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGccacgt                                                  <  1:4119979/100‑3 (MQ=255)                                     gATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGccacca                                                  <  1:1646378/100‑1 (MQ=255)                                        tAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCaagc                                               <  1:620071/100‑3 (MQ=255)                                           tCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAgtttt                                            <  1:976821/100‑1 (MQ=255)                                            cAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAATTTTc                                           <  1:3646151/100‑1 (MQ=255)                                                              tctgccGTTGGTATATTGTTTACCAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTa                         <  1:2797804/96‑1 (MQ=38)                                                                                     cAACATGTTGTAAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAAGTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTc  >  1:5192952/1‑100 (MQ=255)                                                                                                |                                                                                         GTACTCTCTTATGATTAATTGGTGATGTATAACATTGATTAATCAATGCTGTAATCTTACGATAACCGTAGGTATATTGTTTACCAACATGTTGTGAAAATCTATAACTTTCCCCATTTCGATACCATCGCCACCAATTTTTCACTTGTGTTCTATTTCTAATATTTAATTCTTTCATAATTTC  >  NC_007793/62330‑62513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑