Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,843,212	G→T	A82A (GCC→GCA)	SAUSA300_RS14540 ←	cobalt ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,843,212	0	G	T	100.0%	16.4 / NA	8	A82A (GCC→GCA)	SAUSA300_RS14540	cobalt ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/3);  total (5/3)

CCTGTGATGTTAGCGTAAATCGACCAATACCATTGACTAACTCTGTACGCTGATTATAAATCTTCACACCATATTCAGGGTCAAATATGTACACGGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATGAGCATATTTGACCAATAAAATAGA  >  NC_007793/2843118‑2843307                                                                                               |                                                                                                ccTGTGATGTTAGCGTAAATCGACCAATACCATTGACTAACTCTGTACGCTGATTATAAATCTTCACACCATATTCAGGGTCAAATATGTACACTGCaat                                                                                            >  1:1168336/1‑100 (MQ=255)                    tCGACCAATACCATTGACTAACTCTGTACGCTGATTATAAATCTTCACACCATATTCAGGGTCAAATATGTACACTGCAATAATATTTAATATTGTGagc                                                                         <  1:206144/100‑3 (MQ=255)                                 ttGACTAACTCTGTACGCTGATTATAAATCTTCACACCATATTCAGGGTCAAATATGTACACTGCAATAATATTTAATATTGTGAAAAACAGAATGAAtt                                                            >  1:3279080/1‑100 (MQ=255)                                                                tCACACCATATTCAGGGTCAAATATGTACACTGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAat                             >  1:4850411/1‑100 (MQ=255)                                                                   cacCATATTCAGGGTCAAATATGTACACTGCAATAATATTTAATACTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATg                          <  1:1948807/100‑1 (MQ=255)                                                                                        tGTACACTGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATGAGCATATTTGACCAATAAAAt     <  1:2191570/100‑1 (MQ=255)                                                                                        cgTACACTGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATGAGCATATTTGACCAATaaata     >  1:3144665/2‑98 (MQ=255)                                                                                           gcacTGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATGAGCATATTTGACCAATAAAATAGa  >  1:5226387/2‑100 (MQ=255)                                                                                               |                                                                                                CCTGTGATGTTAGCGTAAATCGACCAATACCATTGACTAACTCTGTACGCTGATTATAAATCTTCACACCATATTCAGGGTCAAATATGTACACGGCAATAATATTTAATATTGTGAAAAACAGAATGAATTTAACAACAAAGCGAACTTGTTTCCATTCAATATGAGCATATTTGACCAATAAAATAGA  >  NC_007793/2843118‑2843307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑