Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,830,852	T→C	K556R (AAA→AGA)	SAUSA300_RS14475 ←	lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,830,852	0	T	C	100.0%	94.5 / NA	34	K556R (AAA→AGA)	SAUSA300_RS14475	lipase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/27);  total (7/27)

TACCAGTAATCACAAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTTTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTCAGATCGTAAAATCCATTATCTTTTG  >  NC_007793/2830760‑2830939                                                                                             |                                                                                        tACCAGTAATCACAAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATacac                                                                                  >  1:722440/1‑100 (MQ=255)     aGTAATCACAAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATg                                                                              >  1:3304820/1‑100 (MQ=255)     aGTAATCACAAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATg                                                                              >  1:4552359/1‑100 (MQ=255)             caAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGtt                                                                      <  1:3731953/100‑1 (MQ=255)                                       gCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTg                                            >  1:3023063/1‑100 (MQ=255)                                                  aTCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCa                                 >  1:3668201/1‑100 (MQ=255)                                                        acttAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:4795388/98‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:4796883/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:3987437/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:4019523/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:791680/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:4861601/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:5148580/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:5391772/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:5399805/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:5448883/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:1248603/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:2657370/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:1719665/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:1790014/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:2183240/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:22217/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:2499578/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:3583507/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:2698707/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:293174/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:3408091/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:3422026/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTAAGATTGAAAGAGGGGGAACCCTCACGCGTc                           <  1:4495815/100‑1 (MQ=255)                                                        aTTTAATGCTTTGTGCGTTGCCTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTc                           <  1:324119/100‑1 (MQ=255)                                                         tttAATGCTTTGTGTGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTcg                          <  1:4570997/100‑2 (MQ=255)                                                            aaTGCTTTGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTCAGAt                       >  1:536291/1‑100 (MQ=255)                                                                  ttGTGCGTTGCTTCACCAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTCAGATCGTaaa                 >  1:5307454/1‑100 (MQ=255)                                                                                 ccAGTGTATGTTCTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTCAGATCGTAAAATCCATTATCTTTTg  <  1:2074262/100‑1 (MQ=255)                                                                                             |                                                                                        TACCAGTAATCACAAATGGGAAAAACATATTTAAGTCTGCTTTTTGTCTATCGCTATTTAATGCTTTGTGCGTTGCTTCACCAGTGTATGTTTTATACACAATGTTAGGGTTCAACGACGTTTTACGATTTAAATCTGTTGCACCCTCACGCGTCAGATCGTAAAATCCATTATCTTTTG  >  NC_007793/2830760‑2830939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑