Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,819,073	T→C	D116G (GAT→GGT)	SAUSA300_RS14400 ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,819,073	0	T	C	100.0%	121.4 / NA	43	D116G (GAT→GGT)	SAUSA300_RS14400	flavin reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/39);  total (4/39)

GAATACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACATCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGCCATATTCCACACAAACC  >  NC_007793/2818978‑2819169                                                                                                |                                                                                                 gAATACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGc                                                                                              <  1:3048976/100‑1 (MQ=255)  aaTACGAATCGTTTGAGCGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCa                                                                                             <  1:1448189/100‑1 (MQ=255)  aaTACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCa                                                                                             <  1:4952271/100‑1 (MQ=255)   aTACGAATCGTTTGAACGTACTCACATTCAAAATGAGCTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaa                                                                                            <  1:788791/100‑1 (MQ=255)   aTACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaa                                                                                            <  1:4837248/100‑1 (MQ=255)   aTACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaa                                                                                            <  1:2860097/100‑1 (MQ=255)    tACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaaa                                                                                           <  1:4962603/100‑1 (MQ=255)    tACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaaa                                                                                           <  1:60407/100‑1 (MQ=255)    tACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaaa                                                                                           <  1:2929276/100‑1 (MQ=255)     aCGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCaaaa                                                                                          <  1:1963766/100‑1 (MQ=255)                          acaTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggt                                                                     <  1:2375461/100‑1 (MQ=255)                          acaTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggt                                                                     <  1:4913601/100‑1 (MQ=255)                          acaTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggt                                                                     <  1:3569941/100‑1 (MQ=255)                            aTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggtgg                                                                   <  1:636357/100‑1 (MQ=255)                            aTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggtgg                                                                   <  1:2784887/100‑1 (MQ=255)                            aTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggtgg                                                                   <  1:294987/100‑1 (MQ=255)                            aTTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggtgg                                                                   <  1:3104820/100‑1 (MQ=255)                             ttCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCCTCTGGTGGc                                                                  <  1:299926/100‑1 (MQ=255)                             ttCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTCCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGc                                                                  <  1:4884978/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCtggtgtgc                                                                 <  1:4329962/100‑4 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:4846176/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:5431981/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:4394180/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:3145867/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:411458/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:4014897/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:3842740/100‑1 (MQ=255)                              tCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:608519/100‑1 (MQ=255)                              ctcttccgatcTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCa                                                                 <  1:4407212/90‑1 (MQ=255)                               cAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCag                                                                <  1:2245225/100‑2 (MQ=255)                                aaaaTGAACTGGCGATTCTTTTACACGATAGCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCaag                                                               <  1:1759507/100‑2 (MQ=255)                                 aaaTGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAAtt                                                              <  1:2380471/100‑2 (MQ=255)                                 aaaTGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATg                                                              <  1:707940/100‑1 (MQ=255)                                  tccgatcTGGCGACTCTTTTACACGATATCCTGGAGCTTCTATCCATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGc                                                             <  1:2053034/94‑1 (MQ=255)                                  aaTGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATgt                                                             <  1:5635873/100‑2 (MQ=255)                                       aCTGGCGATTCTTTTGCACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACTCCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCg                                                        <  1:2339118/100‑1 (MQ=255)                                                        ccGATCTCCTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCt                                       <  1:2137623/99‑1 (MQ=255)                                                               ccTGGAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTa                                >  1:402809/1‑100 (MQ=255)                                                                  ggAGCTTCTATACATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAAt                             >  1:22422/1‑100 (MQ=255)                                                                            taCATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGc                   <  1:3193356/100‑1 (MQ=255)                                                                            taCATTTTTCCTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGc                   <  1:1330283/100‑1 (MQ=255)                                                                                     ccTTTGTTACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCTATGATAAATTAACTGCTTCTCTTAAATCATACGTTGCCaaagatcg          >  1:2378218/1‑93 (MQ=255)                                                                                          cttACACCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGCCATATTCCACACaa     >  1:4935848/2‑100 (MQ=255)                                                                                             acacCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGCCTTATTCCACACAaacc  <  1:2870814/100‑1 (MQ=255)                                                                                                |                                                                                                 GAATACGAATCGTTTGAACGTACTCACATTCAAAATGAACTGGCGATTCTTTTACACGATATCCTGGAGCTTCTATACATTTTTCCTTTGTTACATCTGCAAAATTAAATTCATCCTCTTCTGGTGGCAATGCTTTCGATGATAAATTAACTGCTTCTCTTAAATCATACGTTGCCATATTCCACACAAACC  >  NC_007793/2818978‑2819169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑