Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,236	C→T	intergenic (‑28/+543)	SAUSA300_RS00265 ← / ← SAUSA300_RS00270	hypothetical protein/transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,236	0	C	T	100.0%	43.2 / NA	17	intergenic (‑28/+543)	SAUSA300_RS00265/SAUSA300_RS00270	hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

ATAACTCAATGCTGATAAAACGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTACTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAGCTTACGATATTGTAAGTATGTGTTGTTA  >  NC_007793/61143‑61312                                                                                              |                                                                             aTAACTCAATGCTGATAAAACGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTtattattat                                                                        <  1:4847838/100‑1 (MQ=255)       cAATGCTGATAAAACGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAAc                                                                  >  1:1935251/1‑100 (MQ=255)                 aaaaCGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCg                                                        <  1:495771/100‑1 (MQ=255)                     cGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCCCTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCt                                                    <  1:5185718/100‑1 (MQ=255)                                 cATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGtt                                        <  1:3443974/100‑1 (MQ=255)                                    ttttATGCATATTGCTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCATTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTtata                                     <  1:3029195/100‑1 (MQ=255)                                    ttttATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTtata                                     <  1:1485571/100‑1 (MQ=255)                                    ttttATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACCTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTtata                                     <  1:2306365/100‑1 (MQ=255)                                      ttATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAag                                   <  1:3779060/100‑1 (MQ=255)                                       tATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAaga                                  >  1:151879/1‑100 (MQ=255)                                        aTGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAagag                                 >  1:322605/1‑100 (MQ=255)                                         tGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAgatcggaagag                                >  1:5297187/1‑90 (MQ=255)                                          gCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTGTAAGAGaa                               >  1:3256269/1‑100 (MQ=255)                                                               ccACTTTTTTCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAACTTACAATAATGAAAGTAtt          >  1:5680260/1‑99 (MQ=255)                                                                   ttttttCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAACTTACAATAATGAAAGTAttccac      <  1:618158/100‑6 (MQ=255)                                                                    tttttCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAACTTACAATAATGAAAGTAttccact     <  1:874450/100‑7 (MQ=255)                                                                    atcttCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAACTTACAATAATGAAAGTAttccact     <  1:379308/97‑7 (MQ=255)                                                                       ttCACCTTCTTTACTTTTCTTTATTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAACTTACAATAATGAAAGTAttccactaga  >  1:1040747/1‑91 (MQ=255)                                                                                              |                                                                             ATAACTCAATGCTGATAAAACGTAACTATTGTCATTTTTATGCATATTACTATTTGTATTTTCCACTTTTTTCACCTTCTTTACTTTTCTTTACTATTATAAAAACATTAATAGCGAGCTATTACAACAGTTATAAGAGAAGCTTACGATATTGTAAGTATGTGTTGTTA  >  NC_007793/61143‑61312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑