Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,814,794	A→G	F1130S (TTT→TCT)	SAUSA300_RS14395 ←	serine‑rich adhesin for platelets

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,814,794	0	A	G	100.0%	10.9 / NA	6	F1130S (TTT→TCT)	SAUSA300_RS14395	serine‑rich adhesin for platelets
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/2);  total (4/2)

TTGATGTACGTTCTGAATTACTTGTACTTAACGATGTCGAAGTACTTACCGAACCAGATGTGCTCGTAAAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGTTGATAT  >  NC_007793/2814726‑2814876                                                                     |                                                                                   ttGATGTACGTTCTGAATTACTTGTACTTAACGATGTCGAAGTACTTACCGAACCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGc                                                     <  1:1111948/100‑1 (MQ=255)                               aCGATGTCGAAGTACTTACCGAACCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGa                       <  1:5299752/100‑1 (MQ=255)                                               tACCGAACCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGtt       >  1:5542196/1‑100 (MQ=255)                                                   gAACCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGTTGata   >  1:3150824/1‑100 (MQ=255)                                                    aaCCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGTTGatat  >  1:2731276/1‑100 (MQ=255)                                                    aaCCAGATGTGCTCGTAGAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGTTGatat  >  1:5773953/1‑100 (MQ=255)                                                                     |                                                                                   TTGATGTACGTTCTGAATTACTTGTACTTAACGATGTCGAAGTACTTACCGAACCAGATGTGCTCGTAAAAGCACTTTGTGATATTGATTCACTTGATGCAGTTGATGCTGATTTGCTATCACTCGTTGAATCACTAAACGACGTTGATAT  >  NC_007793/2814726‑2814876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑