Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,811,673	G→A	S2170S (TCC→TCT)	SAUSA300_RS14395 ←	serine‑rich adhesin for platelets

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,811,673	0	G	A	100.0%	81.2 / NA	29	S2170S (TCC→TCT)	SAUSA300_RS14395	serine‑rich adhesin for platelets
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/21);  total (8/21)

GTTGCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCGGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTGATTCGGATGTGCTTGTC  >  NC_007793/2811587‑2811769                                                                                       |                                                                                                 gttgCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGGTTCAGATGTACttgttg                                                                                     <  1:1585413/100‑1 (MQ=255) gttgCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACttgttg                                                                                     <  1:2447324/100‑1 (MQ=255) gttgCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACttgttg                                                                                     <  1:4287937/100‑1 (MQ=255) gttgCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACttgttg                                                                                     <  1:3319700/100‑1 (MQ=255)            tGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATa                                                                          >  1:4918109/1‑100 (MQ=255)            tGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATa                                                                          >  1:248453/1‑100 (MQ=255)                 aGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACttgt                                                                     <  1:3832535/100‑1 (MQ=255)                   cGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACttgttg                                                                   <  1:2972729/100‑1 (MQ=255)                         gtgCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTtgttgatgttg                                                             <  1:2445453/100‑1 (MQ=255)                         gtgCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTtgttgatgttg                                                             <  1:3448845/100‑1 (MQ=255)                            atCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGt                                                          >  1:4445424/2‑100 (MQ=255)                               gTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAg                                                       <  1:4876740/100‑1 (MQ=255)                                    gtgGGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTCGATGTTGAGTCAGAAATg                                                  >  1:3368789/1‑100 (MQ=255)                                        gTATGATGCATACTCACTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCtta                                              <  1:4002327/100‑2 (MQ=255)                                          atgatgCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTtgg                                            <  1:1772726/100‑2 (MQ=255)                                                     cgatctggTCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACAt                                 <  1:418512/93‑1 (MQ=255)                                                           gatCTGACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGt                           <  1:2977685/98‑1 (MQ=255)                                                              ccggcGGGTGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACt                        <  1:4546121/96‑1 (MQ=255)                                                                gtcGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCa                      >  1:5423609/3‑100 (MQ=255)                                                                gACGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCa                      >  1:4449506/1‑100 (MQ=255)                                                                 aCGGTTGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAg                     <  1:3833759/100‑1 (MQ=255)                                                                  cGGATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAgg                    <  1:3321867/100‑2 (MQ=255)                                                                   tgATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCTGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTg                   >  1:2863185/2‑100 (MQ=255)                                                                   ggATGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGtt                   <  1:2729802/100‑2 (MQ=255)                                                                     aTGCATTGAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTGAt                 >  1:350973/1‑100 (MQ=255)                                                                            gAGTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTGATTCGGAtg          <  1:2382582/100‑1 (MQ=255)                                                                              gTTAGATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTATGAACCAGACATAGATGTACTCAGTGATTCGGAtggg        <  1:344746/100‑3 (MQ=255)                                                                                  gATTCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTGATTCGGATGTGCTTg    <  1:4877959/100‑1 (MQ=255)                                                                                    ttCAGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGACCCAGACATAGATGTACTCAGTGATTCGGATGTGCTTGTc  <  1:48761/100‑1 (MQ=255)                                                                                       |                                                                                                 GTTGCTTCACTTGATAAGCGAGATGTGCTCGTGCTGTGAGTATGATGCATACTCATTGAGTCTGACGGATGCATTGAGTTAGATTCGGATGTACTTGTTGAGCCGGACATACTTGTTGATGTTGAGTCAGAAATGCTTTGTGAACCAGACATAGATGTACTCAGTGATTCGGATGTGCTTGTC  >  NC_007793/2811587‑2811769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑