Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,791,162	G→A	G555E (GGG→GAG)	SAUSA300_RS14330 →	PTS mannose transporter subunit IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,791,162	0	G	A	100.0%	108.3 / NA	38	G555E (GGG→GAG)	SAUSA300_RS14330	PTS mannose transporter subunit IIABC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/2);  total (36/2)

AAGCAATTGATATGTTGATTCATAAACTTAAAATTTGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGGGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGTTATGAAAAATAATCAT  >  NC_007793/2791069‑2791256                                                                                              |                                                                                               caGCAATTGATATGTTGATTCATAAACTTAAAATTTGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTAc                                                                                          >  1:4137791/2‑100 (MQ=255)              gTTGATTCATAAACTTAAAATTTGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGc                                                                             <  1:1989674/100‑1 (MQ=255)                                  tttGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCcaca                                                         >  1:326134/1‑100 (MQ=255)                                   ttGCCGTTATGTAGAGCACACCTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAACTATTGGCATGAACGTGGCCATTCCACAt                                                        >  1:2501094/1‑100 (MQ=255)                                   ttGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACAt                                                        >  1:1322609/1‑100 (MQ=255)                                   ttGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACAt                                                        >  1:5140450/1‑100 (MQ=255)                                    tGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATg                                                       >  1:4411621/1‑100 (MQ=255)                                    tGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATg                                                       >  1:2049404/1‑100 (MQ=255)                                     gCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGc                                                      >  1:252360/1‑100 (MQ=255)                                     gCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGc                                                      >  1:3306069/1‑100 (MQ=255)                                     gCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGc                                                      >  1:3055349/1‑100 (MQ=255)                                      ccGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCa                                                     >  1:3064571/1‑100 (MQ=255)                                        gTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCaaa                                                   <  1:3182395/100‑1 (MQ=255)                                          tATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGt                                                 >  1:698838/1‑100 (MQ=255)                                             gTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTg                                              >  1:2669184/1‑100 (MQ=255)                                               ctggggcacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAt                                            >  1:1462852/7‑100 (MQ=255)                                                atgtgcacaTCGCATTTAAAAAATGCAATACTAGGACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtg                                           >  1:5391778/6‑100 (MQ=255)                                                 tgtgcacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgt                                          >  1:700856/5‑100 (MQ=255)                                                 tgtgcacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgt                                          >  1:3669378/5‑100 (MQ=255)                                                 cggccacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgt                                          >  1:1746054/5‑100 (MQ=255)                                                  acccacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgtt                                         >  1:5686024/4‑100 (MQ=255)                                                   tgcacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgttg                                        >  1:415660/3‑100 (MQ=255)                                                    acacaTCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGAtgttgt                                       >  1:4081317/1‑100 (MQ=255)                                                                     aaTGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAg                      >  1:1346584/1‑100 (MQ=255)                                                                     aaTGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAg                      >  1:4652083/1‑100 (MQ=255)                                                                      ctGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGc                     >  1:353240/2‑100 (MQ=255)                                                                      aTGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGc                     >  1:3258741/1‑100 (MQ=255)                                                                      aTGCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGCCTGATGTTGTAAAACAACCGATCGTAGc                     >  1:2680156/1‑100 (MQ=255)                                                                        gCAATACTAGAACGTGAAATGGAGTCTACGACATCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTg                   >  1:4633296/1‑100 (MQ=255)                                                                        gCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTg                   >  1:4414914/1‑100 (MQ=255)                                                                        gCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTg                   >  1:3416541/1‑100 (MQ=255)                                                                        gCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTg                   >  1:2929451/1‑100 (MQ=255)                                                                        gCAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTg                   >  1:2076615/1‑100 (MQ=255)                                                                         cAATACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGt                  >  1:4913877/1‑100 (MQ=255)                                                                         aaaTACTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGt                  >  1:2761415/2‑100 (MQ=255)                                                                              gcaGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGTTATGa             >  1:4498516/3‑100 (MQ=255)                                                                              cTAGAACGTGAAATGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGTTATGa             >  1:69587/1‑100 (MQ=255)                                                                                         aaTGGAGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGAAGCTGTTATGAAAAATAATCAt  >  1:479703/1‑100 (MQ=255)                                                                                              |                                                                                               AAGCAATTGATATGTTGATTCATAAACTTAAAATTTGCCGTTATGTAGAGCACACATCGCATTTAAAAAATGCAATACTAGAACGTGAAATGGGGTCTACGACAGCTATTGGCATGAACGTGGCCATTCCACATGCAAAGTCTGATGTTGTAAAACAACCGATTGTAGCTGTTATGAAAAATAATCAT  >  NC_007793/2791069‑2791256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑