Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,753,451	T→G	S148A (TCT→GCT)	SAUSA300_RS14145 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,753,451	0	T	G	100.0%	120.4 / NA	43	S148A (TCT→GCT)	SAUSA300_RS14145	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/2);  total (41/2)

AAGCAGTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAATCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTCAAAT  >  NC_007793/2753359‑2753532                                                                                              |                                                                                  aaGCAGTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGcc                                                                            >  1:5689850/1‑99 (MQ=255) aaGCAGTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCt                                                                            >  1:995426/1‑100 (MQ=255)   gCAGTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTg                                                                          >  1:4656809/1‑100 (MQ=255)     ggTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGAt                                                                        >  1:5027791/2‑100 (MQ=255)               gCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATg                                                              <  1:2778639/100‑1 (MQ=255)                ggcAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGt                                                             >  1:3487935/3‑100 (MQ=255)                cTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGt                                                             <  1:679851/100‑1 (MQ=255)                        attatCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACg                                                     >  1:391705/1‑100 (MQ=255)                                         tGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTa                                    >  1:471393/1‑100 (MQ=255)                                           ctaGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAAc                                  >  1:3737181/3‑100 (MQ=255)                                                   gATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGATGcag                          >  1:948959/1‑98 (MQ=255)                                                          cTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAg                   >  1:1302995/1‑100 (MQ=255)                                                                cTGAAACACAAGAGGCACAAAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTg              >  1:4772160/1‑100 (MQ=255)                                                                 aacgacacAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGag            >  1:3198019/5‑99 (MQ=255)                                                                  taaaCACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaga           >  1:1326902/2‑98 (MQ=255)                                                                  gAAACACAAGAGGCACAAAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGag            >  1:5760530/1‑99 (MQ=255)                                                                  gAAACACAAGAGGCACAAAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaa            >  1:3767582/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGtagat           >  1:3679453/1‑96 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGtagat           >  1:5743504/1‑96 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:948480/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:155817/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:1097787/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:1762394/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:2088234/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:4314880/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:2597056/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:5204840/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:2507108/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGaaa           >  1:210623/1‑100 (MQ=255)                                                                  gAAACACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGAAATGAAGCGGTgtag           >  1:2529164/1‑97 (MQ=255)                                                                  acaaCACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCggcagat           >  1:1130493/3‑95 (MQ=255)                                                                   ttccacaAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAGAt          >  1:120304/4‑100 (MQ=255)                                                                   taaCACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGgagatcg          >  1:5588421/2‑97 (MQ=255)                                                                   caaCACAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAAt          >  1:4669846/2‑100 (MQ=255)                                                                    cacacAAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAAtt         >  1:3329123/2‑100 (MQ=255)                                                                    aaCACAAGAGGCACAAAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAAt          >  1:3002485/1‑100 (MQ=255)                                                                      cacaAGAGGCAC‑AAAAAACTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAAtttt       >  1:900135/1‑100 (MQ=255)                                                                       ccaAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTc      >  1:2837409/2‑100 (MQ=255)                                                                       ccaAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTc      >  1:3884215/2‑100 (MQ=255)                                                                       acaAGAGGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGCGAGAtcgga      >  1:4098910/1‑96 (MQ=255)                                                                          tctggCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAGAtcggaaga   >  1:837461/4‑93 (MQ=255)                                                                          agagGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTCaaa   >  1:3467571/1‑100 (MQ=255)                                                                           gagGCAC‑AAAAAATTAAAGCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTCAAat  >  1:5763032/1‑100 (MQ=255)                                                                                              |                                                                                  AAGCAGTAAAAGATGCTCAAGCTATTATCAATTCATTACTTGAAAGCAGTGATTTAACTGAAGCTGAAACACAAGAGGCAC‑AAAAAATTAAATCTCAGCTTGATCATACACATGTTTATTACGAATGGTTAGAACAGTTAACCGAAGCGATTGAAAGCGGTGAAATTTTCAAAT  >  NC_007793/2753359‑2753532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑