D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,083	T→C	E42E (GAA→GAG)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,083	0	T	C	99.2%	372.1 / NA	133	E42E (GAA→GAG)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (9/122); minor base A (0/1); total (9/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 133 total aligned reads displayed.

ATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATAAAACGTAACTATTGTCATTT  >  NC_007793/61000‑61179

tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTCTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc                                                                                  <  1:1447598/90‑1 (MQ=255)

 tcttccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa                                                                                 <  1:2455282/90‑1 (MQ=255)

  ttccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAt                                                                                <  1:786688/91‑1 (MQ=255)

   tcttccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATa                                                                               <  1:1142155/93‑1 (MQ=255)

   tccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAtg                                                                               <  1:4467611/92‑2 (MQ=255)

    cttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATAc                                                                              <  1:3360591/94‑1 (MQ=255)

     ttccggtCTGCCTATACAAAAACTAATCCATGTCAGAATATGATTTCCTAAAGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                                                                             <  1:4409056/94‑1 (MQ=255)

     ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                                                                             <  1:5224148/95‑1 (MQ=255)

     ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                                                                             <  1:5716041/95‑1 (MQ=255)

     ccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa                                                                             <  1:2334305/94‑1 (MQ=255)

       gatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACaaa                                                                           <  1:2776804/96‑1 (MQ=255)

          aTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATaa                                                                        <  1:3021366/100‑1 (MQ=255)

              gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                                                                    >  1:4033749/2‑100 (MQ=255)

              gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                                                                    >  1:3785813/2‑100 (MQ=255)

              gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt                                                                    <  1:4712518/99‑1 (MQ=255)

              gccTACACAAAAACTAATCCATGTCATAATATGAGTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGGTCATCAGCAAGTATCCATACAAATAAAGGt                                                                    <  1:3935916/99‑1 (MQ=255)

               cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa                                                                   <  1:2432831/93‑1 (MQ=255)

               cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa                                                                   <  1:5467683/93‑1 (MQ=255)

                cggcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTaa                                                                  <  1:3248254/94‑1 (MQ=255)

                      aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc                                                            <  1:4624969/100‑1 (MQ=255)

                      aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc                                                            <  1:5555394/100‑1 (MQ=255)

                          aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt                                                        <  1:249309/100‑1 (MQ=255)

                            tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc                                                      <  1:2022312/100‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATGCAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:2132753/97‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGATAGGTGCg                                                     >  1:2692673/4‑100 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGcgcg                                                     >  1:1708366/4‑100 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:4201379/97‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:4207953/97‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:3301640/97‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:4386860/97‑1 (MQ=255)

                             aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg                                                     <  1:5503437/97‑1 (MQ=255)

                             aatccATGTCATAATATGAGTCCCTAATGCTTTGTTACCATGTCCGGATGTTAGCTCTTCAGCAAGTATCCATACAAATAAAGTTAAAATGACAGGTGCg                                                     <  1:3495627/97‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCgt                                                    <  1:2342875/98‑2 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:2855824/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:2341241/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:5436286/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:1385751/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:5034526/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:4870723/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa                                                    <  1:4822449/98‑1 (MQ=255)

                              atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCAGCAGCAAGTATCCATACAAATAAAGGTAAGATGACAGGTGCGa                                                    <  1:2585414/98‑1 (MQ=255)

                               tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa                                                   <  1:2565557/99‑1 (MQ=255)

                               tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa                                                   <  1:3085020/99‑1 (MQ=255)

                               tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa                                                   <  1:916508/99‑1 (MQ=255)

                               tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa                                                   <  1:3704873/99‑1 (MQ=255)

                                ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa                                                  <  1:5650104/100‑1 (MQ=255)

                                ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa                                                  <  1:5503416/100‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:2652408/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:3515593/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:3409549/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:4337639/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:740667/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:1214596/97‑1 (MQ=255)

                                 catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCACCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa                                                 <  1:100971/97‑1 (MQ=255)

                                                       atgCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGc                           <  1:4804277/100‑1 (MQ=255)

                                                        tgCTTTTTTGCCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCt                          <  1:3391975/100‑1 (MQ=255)

                                                        tgCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCt                          <  1:2621623/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCtt                         <  1:1664368/100‑2 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCta                         <  1:2012041/100‑2 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:2502986/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:5143603/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:5089558/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:4975910/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:4960887/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:878671/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:479860/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:3788418/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:2900481/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:5403035/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:3639657/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:4168017/100‑1 (MQ=255)

                                                         gCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTg                         <  1:4053516/100‑1 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTgt                        <  1:5124025/100‑2 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGa                        <  1:59921/100‑1 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGa                        <  1:4661375/100‑1 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGa                        <  1:2852262/100‑1 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGa                        <  1:1437549/100‑1 (MQ=255)

                                                          cTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGa                        <  1:3777347/100‑1 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGag                       <  1:650317/100‑2 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGag                       <  1:3695819/100‑2 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGAt                       <  1:1071903/100‑1 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGAt                       <  1:5372861/100‑1 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGAt                       <  1:252052/100‑1 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGAt                       <  1:1988241/100‑1 (MQ=255)

                                                           ttttttACCATGTCCTGATGTTGGATGATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGAt                       <  1:4920954/100‑1 (MQ=255)

                                                           ttttttACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTgcc                       <  1:3981727/100‑3 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:4323795/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:179022/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:1952097/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:4007409/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:3589640/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:3040482/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:2472069/100‑1 (MQ=255)

                                                            tttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATa                      <  1:1963546/100‑1 (MQ=255)

                                                             ttttACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCGTACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATaa                     <  1:4937263/100‑1 (MQ=255)

                                                                  ccATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATAAAACGt                <  1:395777/100‑1 (MQ=255)

                                                                     tGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATAAAACGTaaa             <  1:330293/100‑2 (MQ=255)

                                                                          tGAGGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAAAGCTGATAAAACGTAACTAtac        <  1:5463911/100‑3 (MQ=255)

                                                                           gATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCG‑AAAAGGTACTTAAATAACTCAATGCTGATAAAACGTAACTATTGTc      <  1:4138438/100‑1 (MQ=255)

                                                                                tGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGCTACTTAAATAACTCAATGCTGATAAAACGTAACTATTGTCAttt  >  1:955565/1‑100 (MQ=255)

ATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTTAAATAACTCAATGCTGATAAAACGTAACTATTGTCATTT  >  NC_007793/61000‑61179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`