Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,739,999 G→A V212V (GTG→GTA)  SAUSA300_RS14085 → amino acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,739,9990GA100.0% 13.9 / NA 7V212V (GTG→GTA) SAUSA300_RS14085amino acid permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CTCGTATGGAAAATATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTGCCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTCTTAGCGTATAT  >  NC_007793/2739905‑2740094
                                                                                              |                                                                                               
cTCGTATGGAAAATATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTACCaag                                                                                            <  1:3936159/100‑3 (MQ=255)
                                    tAGCTATTATTTTATTTGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGATGACtt                                                        >  1:4223092/1‑100 (MQ=255)
                                      gCTATTATTTTATTTGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTtg                                                      >  1:4508643/1‑100 (MQ=255)
                                                    tGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGc                                        <  1:2995185/100‑1 (MQ=255)
                                                                                      aaCTATGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTCTTAGCGt      <  1:3149186/100‑1 (MQ=255)
                                                                                          aTGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTCTTAGCGTatat  >  1:2348541/1‑100 (MQ=255)
                                                                                          aTGTACCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTCTTAGCGTatat  >  1:3299387/1‑100 (MQ=255)
                                                                                              |                                                                                               
CTCGTATGGAAAATATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATCGTAGGTTTAACAGCAATAAATGTTAGTAACTATGTGCCATTTATTCCAGAACACAAAGTAACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTCTTAGCGTATAT  >  NC_007793/2739905‑2740094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑