Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,733,509	G→T	D551Y (GAT→TAT)	SAUSA300_RS14045 →	acyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,733,509	0	G	T	100.0%	19.7 / NA	9	D551Y (GAT→TAT)	SAUSA300_RS14045	acyl esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/9);  total (0/9)

CTGGCATGAAAACACGTTATGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATGATTCACAATTAATCATTCCTATCGTTAATTGATATGCAGCAATTACGGTCGCTTTTGATTAAAAGTGACATAGTGATAGGACTGTATAACAAG  >  NC_007793/2733432‑2733602                                                                              |                                                                                              ttGGCATGAAAACACGTTATGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTac                                                                         <  1:4635068/99‑2 (MQ=255)                gTTATGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATgc                                                          <  1:2663018/100‑1 (MQ=255)                  tATGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATgcag                                                        <  1:2059806/100‑1 (MQ=255)                   aTGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATgcagc                                                       <  1:3344766/100‑1 (MQ=255)                   aTGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATgcagc                                                       <  1:4073773/100‑1 (MQ=255)                    tGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATgcagca                                                      <  1:4303413/100‑1 (MQ=255)                     gAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATGCAGCaa                                                     <  1:3573938/100‑1 (MQ=255)                                                                cTGGTGGTAAATATTATTCACAATTAATCATTCCTATCGTTAATTGATATGCAGCAATTACGGTCGCTTTTGATTAAAAGTGACATAGTGATAGGACTGt          <  1:2711209/100‑1 (MQ=255)                                                                        aaaTATTATTCACAATTAATCATTCCTATCGTTAATTGATATGCAGCAATTACGGTCGCTTTTGATTAAAAGTGACATAGTGATAGGACTGTATAACAag  <  1:5495406/100‑1 (MQ=255)                                                                              |                                                                                              CTGGCATGAAAACACGTTATGAACATGAAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATGATTCACAATTAATCATTCCTATCGTTAATTGATATGCAGCAATTACGGTCGCTTTTGATTAAAAGTGACATAGTGATAGGACTGTATAACAAG  >  NC_007793/2733432‑2733602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑