Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,732,993	G→C	A379P (GCG→CCG)	SAUSA300_RS14045 →	acyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,732,993	0	G	C	100.0%	22.0 / NA	10	A379P (GCG→CCG)	SAUSA300_RS14045	acyl esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/2);  total (8/2)

CTTTACCTAACGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCGCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGACAAAGATACTGAGTTAGTTGGAAACATGAACT  >  NC_007793/2732916‑2733089                                                                              |                                                                                                 cTTTACCTAACGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGActc                                                                            >  1:2067356/1‑100 (MQ=255)    tACCTAACGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGACTCTGa                                                                         <  1:664834/100‑1 (MQ=255)         aaCGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGACTCTGAAGATa                                                                    >  1:3346492/1‑100 (MQ=255)         aaCGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGACTCTGAAGATa                                                                    >  1:4391013/1‑100 (MQ=255)                aaTATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGACTCTGAAGATAAacaaca                                                             <  1:1305032/100‑1 (MQ=255)                     acacCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATg                                                        >  1:4176204/1‑100 (MQ=255)                                            gaaTCACACATTGAATCATGCAAAGATTAGTAGCCCGCATGTCGCACAATTTGACTCTTAAGATAAACAACAAGATATAAGTTTTAAATATACGTTTGAc                                 >  1:3083826/2‑100 (MQ=255)                                                                        agtagCCCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGACAAAGATACTGAGTTAGTTGGAAACATGa     >  1:3939777/1‑100 (MQ=255)                                                                         ctagCCCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGACAAAGATACTGAGTTAGTTGGAAACATGaa    >  1:628753/2‑100 (MQ=255)                                                                           agCCCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGACAAAGATACTGAGTTAGTTGGAAACATGAACt  >  1:5743269/1‑100 (MQ=255)                                                                              |                                                                                                 CTTTACCTAACGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCGCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGACAAAGATACTGAGTTAGTTGGAAACATGAACT  >  NC_007793/2732916‑2733089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑