Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,726,285	C→T	S47N (AGT→AAT)	SAUSA300_RS14005 ←	type VII secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,726,285	0	C	T	100.0%	52.6 / NA	20	S47N (AGT→AAT)	SAUSA300_RS14005	type VII secretion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/15);  total (5/15)

GGTCAAAAGCCTCACCAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACACTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGAT  >  NC_007793/2726190‑2726380                                                                                                |                                                                                                ggTCAAAAGCCTCTCCAACTTTTTGTATATTATCACTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATg                                                                                             <  1:3135860/100‑1 (MQ=255) ggTCAAAAGCCTCACCAACTTTTTGTATATTATCGATCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATg                                                                                             <  1:1465530/100‑1 (MQ=255)        aGCCTCACCAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCatta                                                                                      >  1:4214440/1‑100 (MQ=255)          ccTCACCAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCatgcag                                                                                    <  1:321396/100‑5 (MQ=255)              cccAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTAttt                                                                                >  1:5249784/2‑100 (MQ=255)                cAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTcc                                                                              <  1:4102336/100‑1 (MQ=255)                   cTTTTTGCATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCAca                                                                           <  1:2716229/100‑2 (MQ=255)                         gTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACtgt                                                                     <  1:260303/100‑1 (MQ=255)                          tataTTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACtgtt                                                                    <  1:1553835/100‑1 (MQ=255)                          tataTTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACtgtt                                                                    <  1:2873011/100‑1 (MQ=255)                           atatTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACtgtct                                                                   <  1:5548032/100‑3 (MQ=255)                            ccgatctgctcgcAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACtgttgt                                                                  <  1:1311616/93‑1 (MQ=255)                             attatCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTa                                                                 <  1:3232472/100‑1 (MQ=255)                                      cgcAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCt                                                        >  1:1230620/1‑100 (MQ=255)                                                 ttGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGttt                                             <  1:5315491/100‑1 (MQ=255)                                                 ttGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGttt                                             <  1:80515/100‑1 (MQ=255)                                                  tGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTc                                            <  1:1062513/100‑1 (MQ=255)                                                   gCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCg                                           <  1:3407609/100‑1 (MQ=255)                                                         acAACAGATTGATTCTTACTCATCAAATCATCTATCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACat                                     >  1:2278501/2‑100 (MQ=255)                                                                                          aTCACATTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTACCCGGTTTCGACACATTATTACATGTTTCAACTGCGCTTTTAATAgatc    >  1:5106794/1‑99 (MQ=255)                                                                                            aacatTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAgatcgg  >  1:440659/2‑97 (MQ=255)                                                                                                |                                                                                                GGTCAAAAGCCTCACCAACTTTTTGTATATTATCGCTCGCAGTTCGTATTGCTTCAGCAACAGATTGATTCTTACTCATCAAATCATCTATCACACTATGAGCATTATTATTTCCACTTACTGTTGTACTTTCATCTTTATCCGGTTTCGACACATTATTACATGTTTCAACAGCGCTTTTAATAGATGAT  >  NC_007793/2726190‑2726380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑