Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,718,483	G→A	M41I (ATG→ATA)	SAUSA300_RS13960 →	TetR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,718,483	0	G	A	100.0%	35.5 / NA	14	M41I (ATG→ATA)	SAUSA300_RS13960	TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/0);  total (14/0)

AATAGCGCACAAACTTTTTGATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGTATGGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCATTTTAAGCAA  >  NC_007793/2718402‑2718576                                                                                  |                                                                                              aaTAGCGCACAAACTTTTTGATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGTATAGGGACATTGTATCGACAt                                                                             >  1:445530/1‑100 (MQ=255)       gcACAAACTTTTTGATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGTATAGGGACATTGTATCGACATTTCaaa                                                                       >  1:1541538/1‑100 (MQ=255)                         ctaagGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGAATAGGGACATCGTATCGTCATTTCAAAGATAAAAGCGATTTATGc                                                     >  1:3829024/3‑100 (MQ=255)                                                        aTTGCTAAAGAACTAGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATAttt                      >  1:1225561/1‑100 (MQ=255)                                                              aaaGCACTAGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCa                >  1:1673404/1‑100 (MQ=255)                                                              aaaGAACTAGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCa                >  1:2965081/1‑100 (MQ=255)                                                              aaaGAACTAGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCa                >  1:4025551/1‑100 (MQ=255)                                                                  aaCTAGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCa            >  1:2153290/1‑100 (MQ=255)                                                                      aGGTATCGGTATAGGGGCATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCAtttt        >  1:4327055/1‑100 (MQ=255)                                                                      aGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCAtttt        >  1:3506360/1‑100 (MQ=255)                                                                      aGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCAtttt        >  1:4165691/1‑100 (MQ=255)                                                                      aGGTATCGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCAtttt        >  1:4954354/1‑100 (MQ=255)                                                                        ctgctggTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCCTACAAAGAGACCTTGATATTTTTATCACTCATTTTaa      >  1:4007693/6‑100 (MQ=255)                                                                            cGGTATAGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCATTTTAAGCaa  >  1:1571898/1‑100 (MQ=255)                                                                                  |                                                                                              AATAGCGCACAAACTTTTTGATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGTATGGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAAAGAGACCTTGATATTTTTATCACTCATTTTAAGCAA  >  NC_007793/2718402‑2718576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑