Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,717,834	C→T	W26* (TGG→TGA)	SAUSA300_RS13950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,717,834	0	C	T	100.0%	58.0 / NA	22	W26* (TGG→TGA)	SAUSA300_RS13950	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/21);  total (1/21)

CAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATACCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCTATTACTAAAATATCTTTCATTT  >  NC_007793/2717747‑2717913                                                                                        |                                                                                cAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCctct                                                                     <  1:4164445/100‑1 (MQ=255)       aCCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCctcccctatg                                                               <  1:852058/100‑8 (MQ=255)        tcTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCGTCCTCTAACAACt                                                              <  1:3639579/99‑1 (MQ=255)         cTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTg                                                             <  1:2646423/100‑1 (MQ=255)             aaCGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGtttt                                                         <  1:3920408/100‑1 (MQ=255)                                tGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGca                                      <  1:426128/100‑2 (MQ=255)                                    cTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTccc                                  <  1:1506823/100‑1 (MQ=255)                                       tGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGt                               <  1:5567513/100‑1 (MQ=255)                                       tGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGt                               <  1:4579176/100‑1 (MQ=255)                                        gTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGtg                              <  1:881215/100‑2 (MQ=255)                                         tCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGtgg                             <  1:705370/100‑3 (MQ=255)                                         tCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTg                             <  1:3788873/100‑1 (MQ=255)                                           tGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATGCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGtgcag                           <  1:3888772/100‑5 (MQ=255)                                           tGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTgcg                           <  1:5537239/100‑1 (MQ=255)                                           tGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTgcg                           >  1:812373/1‑100 (MQ=255)                                             aaaGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGcc                         <  1:4460172/100‑1 (MQ=255)                                             aaaGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGcc                         <  1:2488472/100‑1 (MQ=255)                                              aaGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCt                        <  1:357172/100‑1 (MQ=255)                                              aaGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCt                        <  1:1174441/100‑1 (MQ=255)                                                 tttACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCTAtt                     <  1:3548321/100‑1 (MQ=255)                                                 tttACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCTAtc                     <  1:1708595/100‑2 (MQ=255)                                                                    cTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCTATTACTAAAATATCTTTCAttt  <  1:1295189/100‑1 (MQ=255)                                                                                        |                                                                                CAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATACCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCTATTACTAAAATATCTTTCATTT  >  NC_007793/2717747‑2717913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑