Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,714,599	C→T	S12L (TCA→TTA)	SAUSA300_RS13925 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,714,599	0	C	T	100.0%	14.7 / NA	7	S12L (TCA→TTA)	SAUSA300_RS13925	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

TTTAGGCTAGCAGTATCATCTATAACTCAAGACTATGAAAAATAGTATATCACAAAATTCTGAATTTTCAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACTTTTATTTTATCTTCATTTTTAAAATCCATTAATACAAT  >  NC_007793/2714531‑2714683                                                                     |                                                                                     actAGGCTAGCAGTATCATCTATAACTCAAGACTATGAAAAATAGTATATCACAAAATTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACata                                                       >  1:2029788/3‑100 (MQ=255)         aGCAGTATCATCTATAACTCAAGACTATGAATAATAGTATATCACACAATTCAGAATTTTTAGATAAAGCAATTGGCAATTTTTTAAACATATTGTTACa                                               >  1:2020649/1‑100 (MQ=255)         aGCAGTATCATCTATAACTCAAGACTATGAAAAATAGCATATCACAAAATTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACa                                               >  1:4428635/1‑100 (MQ=255)                      ataACTCAAGACTATGAAAAATAGTATATCACAAAATTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACttttatt                                  >  1:2130701/1‑100 (MQ=255)                        aaCTCAAGACTATGAAAAATAGTATATCACAAAATTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACttttatttt                                >  1:1526290/1‑100 (MQ=255)                                            aGTATATCACAAAATTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACTTTTATTTTATCTTCATTTTTAAAATCCa            >  1:272487/1‑100 (MQ=255)                                                      aaaaTTCTGAATTTTTAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACTTTTATTTTATCTTCATTTTTAAAATCCATTAATACAAt  <  1:5005478/100‑1 (MQ=255)                                                                     |                                                                                     TTTAGGCTAGCAGTATCATCTATAACTCAAGACTATGAAAAATAGTATATCACAAAATTCTGAATTTTCAGATAAAGAAATTGGCAATTTTTCAAACATATTGTTACAATACACTTTTATTTTATCTTCATTTTTAAAATCCATTAATACAAT  >  NC_007793/2714531‑2714683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑