Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,432,074	(A)6→7	coding (216/315 nt)	SAUSA300_RS12495 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,432,068	1	.	A	100.0%	11.4 / NA	6	I70I (ATC→ATA)	SAUSA300_RS12495	transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (1/5);  total (1/5)

ATAAAACTATTAATCTTTTTAGAGTGAATCCTAATACTGATGCGCTAATTGAATCTTTATTTATTCAGTTTCATAATAAAATGCATATC‑AAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACCTCTTTATCTACGC  >  NC_007793/2431980‑2432155                                                                                          |                                                                                        ataAAACTATTAATCTTTTTAGAGTGAATCCTAATACTGATGCGCTAATTGAATCTTTATTTATTCAGTTTCATAATAAAATGCATATC‑aaaaaaagctt                                                                              <  1:3548050/100‑6 (MQ=255)                                                              tattCAGTTTCATAATAAAATGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTcctg                  <  1:5207038/100‑3 (MQ=255)                                                                ttCAGTTTCATAATAAAATGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTccccta                <  1:5401767/100‑4 (MQ=255)                                                                      ttCATAATAAAATGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACctctgg          <  1:904832/100‑3 (MQ=255)                                                                          taataaAATGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACCTCTTTATCt      <  1:1319332/100‑1 (MQ=255)                                                                              aaaaTGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACCTCTTTATCTACgg  <  1:2121023/100‑2 (MQ=255)                                                                              aaaaTGCATATCAAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACCTCTTTATCTACGc  >  1:585874/1‑100 (MQ=255)                                                                                          |                                                                                        ATAAAACTATTAATCTTTTTAGAGTGAATCCTAATACTGATGCGCTAATTGAATCTTTATTTATTCAGTTTCATAATAAAATGCATATC‑AAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTCATTGCCTAAAAACTTAATGTCCCGACCTCTTTATCTACGC  >  NC_007793/2431980‑2432155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑