Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,536,960	(A)6→7	intergenic (‑19/+106)	SAUSA300_RS07480 ← / ← SAUSA300_RS07485	hypothetical protein/elastin‑binding protein EbpS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,536,954	1	.	A	100.0%	12.2 / NA	6	intergenic (‑13/+112)	SAUSA300_RS07480/SAUSA300_RS07485	hypothetical protein/elastin‑binding protein EbpS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (1/5);  total (1/5)

TCTTTTTGATGACTATTTAAAAAGTTAAATTGATATGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATG‑AAAAAACCGCTACATACTTATAAGCGCAATTTAAGTTCTCCAAAATATTTAG  >  NC_007793/1536871‑1537006                                                                                     |                                                     tCTTTTTGATGACTATTTAAAAAGTTAAATTGATATGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATGAAAAAAACCGCTACAt                                       <  1:2079822/100‑1 (MQ=255) ccgatctgatgaCTATTTAAAAAGTTAAATTGATATGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATGAAAAAAACCGCTACAt                                       <  1:2971350/94‑1 (MQ=255)             cTATTTAAAAGGTTAAATTGATATGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATGAAAAAAACCGCTACATACTTATAAgcgc                           <  1:1065556/100‑1 (MQ=255)                                    tGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATGAAAAAAACCGCTACATACTTATAAGCGCAATTTAAGTTCTCCAAAatatgg    <  1:5164643/100‑3 (MQ=255)                                     tccAATATTCGAGAAAAGACCTTGTTAAATAACAGACTTTGAAAAATGAAAAAAACCGCTACATACTTATAAGCGCAATTTAAGTTCTCCAAAATATTTa   >  1:1911421/2‑100 (MQ=255)                                      ccAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATGAAAAAAACCGCTACATACTTATAAGCGCAATTTAAGTTCTCCAAAATATTTag  <  1:1270545/100‑1 (MQ=255)                                                                                     |                                                     TCTTTTTGATGACTATTTAAAAAGTTAAATTGATATGCCAATATTCGAGAAAAGACCTTGTTAAATAACAAACTTTGAAAAATG‑AAAAAACCGCTACATACTTATAAGCGCAATTTAAGTTCTCCAAAATATTTAG  >  NC_007793/1536871‑1537006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑