Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,354,819	(G)5→6	intergenic (+170/+210)	SAUSA300_RS06705 → / ← SAUSA300_RS06710	secretion protein/LexA repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,354,814	1	.	G	100.0%	43.9 / NA	18	intergenic (+165/+215)	SAUSA300_RS06705/SAUSA300_RS06710	secretion protein/LexA repressor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/8);  total (10/8)

TAAACCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑GGGGGCGAGTTTTAATTTGTGTAACATTTTGCAGAAATCTGTAATTCAAAATTTATATTAAAGAATGAGATTATCTAACAATTCGCAATTTCTG  >  NC_007793/1354718‑1354908                                                                                                  |                                                                                               caaaCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑ggg                                                                                             >  1:3536591/2‑100 (MQ=255)  aaaCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑gggg                                                                                            >  1:4787125/1‑100 (MQ=255)   aaCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑ggggg                                                                                           >  1:5753461/1‑100 (MQ=255)   aaCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑ggggg                                                                                           >  1:2348854/1‑100 (MQ=255)    aCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑gggggg                                                                                          >  1:2366343/1‑99 (MQ=255)    aCCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑gggggg                                                                                          >  1:335747/1‑99 (MQ=255)            gtGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGtttt                                                                                   >  1:2623822/2‑100 (MQ=255)                     taCTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTgtgt                                                                          <  1:1307348/100‑1 (MQ=255)                         tGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACa                                                                      >  1:3146688/1‑100 (MQ=255)                         tGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACa                                                                      >  1:5474419/1‑100 (MQ=255)                         tGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACa                                                                      >  1:248839/1‑100 (MQ=255)                         tGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACa                                                                      >  1:1639065/1‑100 (MQ=255)                         acgaCGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACa                                                                      >  1:706789/4‑100 (MQ=255)                          gAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACAt                                                                     >  1:2667749/1‑100 (MQ=255)                           aaCGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACAtt                                                                    >  1:3427169/1‑100 (MQ=255)                               cgTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGc                                                                <  1:3267565/100‑1 (MQ=255)                               cgTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGc                                                                <  1:367291/100‑1 (MQ=255)                               cgTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGc                                                                <  1:2324826/100‑1 (MQ=255)                               cgTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGc                                                                <  1:1753052/100‑1 (MQ=255)                                 tCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGCAg                                                              <  1:1114232/100‑1 (MQ=255)                                     aGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGCAggata                                                          <  1:2781388/100‑5 (MQ=255)                                                                  aataataatGGCTCGCTCCTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGCAGAAATCTGTAATTCAAAATTTATATTAAAGAATg                             <  1:2725740/100‑1 (MQ=255)                                                                                   ccTGTAAATTATTACGGGGGGCGAGTTTTAATTTGTGTAACATTTTGCAGAAATCTGTAATTCAAAATTTATATTAAAGAATGAGATTATCTAACAATTc            >  1:3712746/1‑100 (MQ=255)                                                                                             attaCGGGGGGCGAGTTTTAATTTGTGTAACATTTTGCAGAAATCTGTAATTCAAAATTTATATTAAAGAATGAGATTATCTAACAATTCGCAATTTCTg  >  1:4824492/1‑100 (MQ=255)                                                                                                  |                                                                                               TAAACCAAAAGATGCGGATATACTTGAACGCGTCAAAGATATACTTAATAAAAAAGAAAGAAAAAAATAATAATGGCTCGCTCCTGTAAATTATTAC‑GGGGGCGAGTTTTAATTTGTGTAACATTTTGCAGAAATCTGTAATTCAAAATTTATATTAAAGAATGAGATTATCTAACAATTCGCAATTTCTG  >  NC_007793/1354718‑1354908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑