Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	730,913	+G	coding (1953/1980 nt)	SAUSA300_RS03500 →	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	730,913	1	.	G	100.0%	67.4 / NA	27	D651E (GAC→GAG)	SAUSA300_RS03500	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/3);  total (24/3)

CGAGACAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAC‑AAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTA  >  NC_007793/730814‑731004                                                                                                      |                                                                                            tgagaCAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAc                                                                                              >  1:4114562/2‑100 (MQ=255) cGAGACAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAc                                                                                              >  1:3604712/1‑100 (MQ=255)   agaCAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAcga                                                                                            >  1:776653/1‑98 (MQ=255)          tATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGt                                                                                     >  1:527351/1‑100 (MQ=255)              aGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCa                                                                                 <  1:57397/100‑1 (MQ=255)                    gttGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAATCGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTa                                                                           >  1:1106559/2‑100 (MQ=255)                     ctGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGACAACATTaa                                                                          >  1:4286012/2‑100 (MQ=255)                       gAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATc                                                                        >  1:5732636/1‑100 (MQ=255)                                     ttATATAAATTTTAAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTaa                                                           <  1:2908296/100‑1 (MQ=255)                                             aTTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAag                                                  <  1:4479293/100‑1 (MQ=255)                                              ggtt‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTATTAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAaga                                                 >  1:830556/3‑100 (MQ=255)                                                                            cccTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACt                    >  1:662172/3‑100 (MQ=255)                                                                                ccgggTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:4549440/5‑100 (MQ=255)                                                                                aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:5606487/1‑100 (MQ=255)                                                                                aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:3898717/1‑100 (MQ=255)                                                                                aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:3759293/1‑100 (MQ=255)                                                                                aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:3178289/1‑100 (MQ=255)                                                                                aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                >  1:799979/1‑100 (MQ=255)                                                                                   tGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGGGATAAATATGAATGGAACCCTAGTTTACTATCTaaa             >  1:4200601/1‑100 (MQ=255)                                                                                   tGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTaaa             >  1:2360271/1‑100 (MQ=255)                                                                                        tAGAAACATTGGACGAAAATAGTGGCAGCATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGa        >  1:210239/1‑100 (MQ=255)                                                                                        caGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGa        >  1:2924007/2‑100 (MQ=255)                                                                                         aGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGAc       >  1:4189164/1‑100 (MQ=255)                                                                                         aGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGAc       >  1:84496/1‑100 (MQ=255)                                                                                          gAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACa      >  1:872649/1‑100 (MQ=255)                                                                                          caaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACa      >  1:970306/2‑100 (MQ=255)                                                                                           aaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATCTGAATGGAACCCTAGTTTACTATCTAAAATTGACaa     >  1:4661576/1‑100 (MQ=255)                                                                                           aaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACaa     >  1:5574423/1‑100 (MQ=255)                                                                                           aaaCATTGGACGAAAATAGTGGCAACATTAACCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACaa     >  1:3211753/1‑100 (MQ=255)                                                                                              cATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTa  >  1:2726552/1‑100 (MQ=255)                                                                                                      |                                                                                            CGAGACAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTT‑AAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAC‑AAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTA  >  NC_007793/730814‑731004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑