Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	670,897	(T)7→8	intergenic (+64/‑324)	argS → / → SAUSA300_RS03210	arginine‑‑tRNA ligase/endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	670,890	1	.	T	100.0%	12.4 / NA	6	intergenic (+57/‑331)	argS/SAUSA300_RS03210	arginine‑‑tRNA ligase/endonuclease III
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (3/3);  total (3/3)

GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑TTTTTTTAGGTATGTGACATATTGGGGAATGCTTAGTATGTGAATAAGGTTAAGAGGAACACAGTTGGA  >  NC_007793/670792‑670959                                                                                                    |                                                                      gCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAgtg‑t                                                                      >  1:5281813/1‑100 (MQ=255)  ccttGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tt                                                                     >  1:2971972/3‑100 (MQ=255)   cctGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttt                                                                    >  1:4064728/3‑100 (MQ=255)   aTTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttt                                                                    >  1:4188440/1‑100 (MQ=255)   aTTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttt                                                                    >  1:3826665/1‑100 (MQ=255)    ttGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttt                                                                   >  1:1374720/1‑100 (MQ=255)    ttGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttt                                                                   >  1:4512345/1‑100 (MQ=255)    ctGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttt                                                                   >  1:230344/2‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:1260295/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:5183135/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:4919394/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:4621543/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:2533766/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:2525341/1‑100 (MQ=255)     tGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTCCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:2741181/1‑100 (MQ=255)     tGGCAATGGTCGGTGCAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑ttttt                                                                  >  1:1813935/1‑100 (MQ=255)      ggCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttttt                                                                 >  1:19991/1‑100 (MQ=255)      ggCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttttt                                                                 >  1:5767476/1‑100 (MQ=255)      ggCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCCCGAAAGATACTTTGTGTTGGAGTG‑tttttt                                                                 >  1:5357683/1‑100 (MQ=255)      ggCAATGGTCGGTGTAAGCGCACCTGAATCAATGCAAGAACATTTATATACACTCCAACGCAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttttt                                                                 >  1:937679/1‑100 (MQ=255)        aaaTGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑tttttttt                                                               >  1:4957722/2‑99 (MQ=255)                ggTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTAtgg                                                        <  1:4320716/100‑2 (MQ=255)                     taGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTATGTGACat                                                   >  1:103489/2‑100 (MQ=255)                       gcgcACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTATGTGACatat                                                 >  1:267231/1‑100 (MQ=255)                        ggcACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTATGTGACATAtt                                                >  1:4910320/2‑100 (MQ=255)                          cACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTATGTGACATATTgg                                              <  1:5050815/100‑1 (MQ=255)                                                                      gTTTCTCGAAAGATACTTTGTGTTGGAGTGTTTTTTTTAGGTATGTGACATATTGGGGAATGCTTAGTATGTGAATAAGGTTAAGAGGAACACAGTTggg  <  1:4961781/100‑2 (MQ=255)                                                                                                    |                                                                      GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAAGAACATTTATATACACTCCAACGTAGAGTTTCTCGAAAGATACTTTGTGTTGGAGTG‑TTTTTTTAGGTATGTGACATATTGGGGAATGCTTAGTATGTGAATAAGGTTAAGAGGAACACAGTTGGA  >  NC_007793/670792‑670959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑