Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	212,492	(T)6→7	intergenic (‑123/+132)	SAUSA300_RS00960 ← / ← SAUSA300_RS00965	hypothetical protein/acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	212,486	1	.	T	100.0%	36.5 / NA	14	intergenic (‑117/+138)	SAUSA300_RS00960/SAUSA300_RS00965	hypothetical protein/acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (3/11);  total (3/11)

GTGAAAATAATCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAG‑TTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACGAGATGAACGGTAAATAAATGTTTTAGCGCAATGG  >  NC_007793/212413‑212567                                                                           |                                                                                  gtgAAAATAATCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGt                                                          <  1:5558190/100‑1 (MQ=255)         aatCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTAtt                                                  >  1:3414683/1‑100 (MQ=255)          atCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTc                                                 <  1:5208945/100‑1 (MQ=255)           tCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCa                                                <  1:1251829/100‑1 (MQ=255)           ctacaATAAAAAGCTTTGCAAAACTTAAAATATTTTGACAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCa                                                >  1:5740693/3‑100 (MQ=255)            cacaAT‑AAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCaaa                                              <  1:1371110/100‑1 (MQ=255)                       gCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACg                                    <  1:1930845/100‑1 (MQ=255)                       gCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACg                                    <  1:5404396/100‑1 (MQ=255)                           tGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACgagag                                <  1:5373987/100‑2 (MQ=255)                           tGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACgagaa                                >  1:1901931/1‑99 (MQ=255)                                               gATAAACAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACGAGATGAACGGTAAATAAATGtttt            <  1:3963273/100‑1 (MQ=255)                                                   aaCAAATTTGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACGAGATGAACGGTAAATAAATGTTTTAgct        <  1:4317163/100‑2 (MQ=255)                                                       aaTTTGTAAAAAGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAAGTGTTAACGAGATGAACGGTAAATAAATGTTTTAgcgcctg    <  1:5680205/100‑4 (MQ=255)                                                         tttGTAAAAGGTTATTAGTTTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACGAGATGAACGGTAAATAAATGTTTTAGCGCAATgg  <  1:4803149/100‑1 (MQ=255)                                                                           |                                                                                  GTGAAAATAATCACAATAAAAAGCTTTGCAAAACTTAAAATATTTTGATAAACAAATTTGTAAAAGGTTATTAG‑TTTTTTACATCTCATATTGTATCGTACTGTATTCAAAATTGTTAACGAGATGAACGGTAAATAAATGTTTTAGCGCAATGG  >  NC_007793/212413‑212567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑