Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	62,659	+A	intergenic (‑71/+441)	SAUSA300_RS00270 ← / ← SAUSA300_RS00275	transposase/spermidine N1‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	62,658	1	.	A	100.0%	12.0 / NA	6	intergenic (‑70/+442)	SAUSA300_RS00270/SAUSA300_RS00275	transposase/spermidine N1‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/6);  total (0/6)

TGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTCAGTCTAAAATTAAA  >  NC_007793/62559‑62681                                                                                                     |                        tGTTTCTACTGAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACt                          <  1:320370/100‑1 (MQ=255)           gAATAAGAAACTCTTTTCATAGAAAAAAACGCCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAACTGGGGTc                <  1:3156216/100‑1 (MQ=255)           gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc                <  1:1391135/100‑1 (MQ=255)           gAATAAGAAACTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTc                <  1:3995734/100‑1 (MQ=255)                     ctctTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacata      <  1:3907763/100‑5 (MQ=255)                     ctctTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacacc      <  1:3553785/100‑5 (MQ=255)                         tttCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAATGTTTTTATATAATTCCCACTAATTGGGGTCAGTCtacaggccac  <  1:4450438/100‑9 (MQ=255)                                                                                                     |                        TGTTTCTACTGAATAAGAAGCTCTTTTCATAGAAAAAAACACCTCCGTATGATTCATTTTAATATGAATTCAACGAAAGTGTTTTTATATAATTCCCACT‑ATTGGGGTCAGTCTAAAATTAAA  >  NC_007793/62559‑62681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑