Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,347,247	(A)8→7	coding (1293/1407 nt)	SAUSA300_RS11955 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,347,240	0	A	.	100.0%	279.2 / NA	72	coding (1300/1407 nt)	SAUSA300_RS11955	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/72);  total (0/72)

GTATTTGACTTCTATACTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAGAAAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTAA  >  NC_007793/2347142‑2347327                                                                                                   |                                                                                        gTATTTGACTTCTATACTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAGaa                                                                                        <  1:868832/100‑1 (MQ=255)              attcTTTTGAAAAAATTTTCTATATCATCTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AACAAAAGTATTGcc                                                                          <  1:1894050/97‑1 (MQ=255)               taCTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCt                                                                         <  1:4781072/100‑1 (MQ=255)               taCTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCt                                                                         <  1:4502819/100‑1 (MQ=255)               taCTTTTGAAAAAATTTTCTATATCAACTTTAGCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCt                                                                         <  1:5112190/100‑1 (MQ=255)                aCTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCtt                                                                        <  1:4393000/100‑1 (MQ=255)                aCTTTTGAAAAAATTTTCTATATCAACTTTACCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCtt                                                                        <  1:1880358/100‑1 (MQ=255)                 cTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTc                                                                       <  1:535957/100‑1 (MQ=255)                     tGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACtt                                                                   <  1:685172/100‑1 (MQ=255)                     tGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACtt                                                                   <  1:1894564/100‑1 (MQ=255)                     tGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACtg                                                                   <  1:5576894/100‑2 (MQ=255)                     tGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTCTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACtt                                                                   <  1:5372974/100‑1 (MQ=255)                        gaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:2981216/99‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTaat                                                                <  1:4167492/100‑3 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4418127/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4511192/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4525785/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4414824/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:5305575/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4604026/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4668972/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:524945/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1064875/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:5592287/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:699574/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:711418/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:894174/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:938648/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:355958/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1064591/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1327633/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1468712/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:147402/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:329900/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1766573/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1960585/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:2221208/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACTTTATCCATATTTAAAATAGCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:4264674/100‑1 (MQ=255)                        aaaaaTTTTCTATATCAACCTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATc                                                                <  1:1450533/100‑1 (MQ=255)                         acaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCa                                                               <  1:3203150/98‑1 (MQ=255)                         aaaaTTTTCTTTATCAACTTTATCCAGATATAAAATATCTTTATTGAACACACAATGCAAATCTGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTAGCa                                                               <  1:4748925/100‑1 (MQ=255)                         aaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTaagc                                                               <  1:4148574/100‑4 (MQ=255)                         aaaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATct                                                               <  1:3979816/100‑2 (MQ=255)                          aaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAg                                                              <  1:849093/100‑1 (MQ=255)                          aaaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAg                                                              <  1:734773/100‑1 (MQ=255)                           aaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAgg                                                             <  1:718043/100‑2 (MQ=255)                           aaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAgg                                                             <  1:5664598/100‑2 (MQ=255)                           aaTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGt                                                             <  1:1829031/100‑1 (MQ=255)                            aTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAGCACTCAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGtt                                                            <  1:538708/100‑1 (MQ=255)                            aTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAggg                                                            <  1:4269330/100‑3 (MQ=255)                            aTTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGtc                                                            <  1:2541831/100‑2 (MQ=255)                             ttttCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTg                                                           <  1:3557507/100‑1 (MQ=255)                             ttttCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTg                                                           <  1:2078379/100‑1 (MQ=255)                             ttttCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTCGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGtga                                                           <  1:3085018/100‑3 (MQ=255)                             ttttCTATATCAACTTTATCCATATTTAAAATACCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTg                                                           <  1:3435049/100‑1 (MQ=255)                              tttCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGtgtg                                                          <  1:5743822/100‑4 (MQ=255)                               ttCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGtcccc                                                         <  1:2112309/100‑5 (MQ=255)                                      tCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAAc                                                  <  1:3215991/100‑1 (MQ=255)                                                            tatCTTTATTGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAaataaata                            <  1:3091600/100‑1 (MQ=255)                                                                    ttGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATAcc                    <  1:4015175/100‑1 (MQ=255)                                                                    ttGAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATAcc                    <  1:987894/100‑1 (MQ=255)                                                                      gAACACACAATGCAAATATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACcaa                  <  1:64224/100‑2 (MQ=255)                                                                                    aaTATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCt    <  1:5513236/100‑1 (MQ=255)                                                                                    aaTATGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCt    <  1:1912341/100‑1 (MQ=255)                                                                                    aaTATGGTTTTGCAG‑‑AAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:4175075/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:5466948/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:5455711/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:5398772/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:5275949/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:2029057/100‑1 (MQ=255)                                                                                     atatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTa   <  1:1326029/100‑1 (MQ=255)                                                                                      tatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTag  <  1:154055/100‑2 (MQ=255)                                                                                      tatGGTTTTGCAG‑AAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTag  <  1:4908810/100‑2 (MQ=255)                                                                                                   |                                                                                        GTATTTGACTTCTATACTTTTGAAAAAATTTTCTATATCAACTTTATCCATATTTAAAATATCTTTATTGAACACACAATGCAAATATGGTTTTGCAGAAAAAAAAGTATTGCCTTCACTTATCAGTTGATAATTAACATCCTTAAAATTTAAATAAATAATAATACCATCATTTTCTTTGGCTAA  >  NC_007793/2347142‑2347327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑