| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,962,436 | (A)7→6 | intergenic (‑48/‑46) | SAUSA300_RS09740 ← / → SAUSA300_RS09745 | alpha/beta hydrolase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,962,430 | 0 | A | . | 94.1% | 52.1 / ‑2.0 | 17 | intergenic (‑42/‑52) | SAUSA300_RS09740/SAUSA300_RS09745 | alpha/beta hydrolase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (4/12); minor base T (0/1); total (4/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01 | |||||||||||
TTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCAT > NC_007793/1962342‑1962526 | tttCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTg < 1:2266313/100‑1 (MQ=255) ctactaTTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGAtt < 1:592299/100‑1 (MQ=255) tactaTTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTa < 1:4924891/100‑1 (MQ=255) taTTTCAATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCt < 1:320067/100‑1 (MQ=255) aTTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCta < 1:73312/100‑1 (MQ=255) tttCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCtaa < 1:1020157/100‑2 (MQ=255) aTAGAATGTATAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGtagc < 1:2418135/100‑3 (MQ=255) tcttccgaTCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCtc < 1:1604468/93‑2 (MQ=255) aatTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTa < 1:5172450/100‑1 (MQ=255) tACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAAtt < 1:5249534/100‑1 (MQ=255) cTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAggca < 1:365184/100‑4 (MQ=255) gctCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAAt > 1:773709/3‑100 (MQ=255) gTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATg > 1:3949227/1‑100 (MQ=255) ctcttccgatctctcCTTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGt < 1:1116086/91‑1 (MQ=255) ctcCCTTCAACTTACTACTTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAg > 1:4889245/1‑100 (MQ=255) actactTCTTTTCTATTTTT‑AAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAAt > 1:3527164/1‑100 (MQ=255) ttttTAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGtct < 1:1221257/100‑2 (MQ=255) ttttAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGtcat < 1:2330415/100‑1 (MQ=255) | TTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCAT > NC_007793/1962342‑1962526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |