Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	973,979	(T)6→5	intergenic (‑73/‑170)	SAUSA300_RS04780 ← / → SAUSA300_RS04785	hypothetical protein/peptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	973,974	0	T	.	100.0%	17.2 / NA	6	intergenic (‑68/‑175)	SAUSA300_RS04780/SAUSA300_RS04785	hypothetical protein/peptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/2);  total (4/2)

ATTTTTTCAACATTTGATCACTCCCACTTATAGAAAACTCTTACGCATAGTTTACATTAAAATCAGACATTGAGGAATGATTTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAATCAATCATTCTTCATTAGTTTAAAGCAAAAAAATATTGATAT  >  NC_007793/973894‑974051                                                                                 |                                                                              aTTTTTTCAACATTTGATCACTCCCACTTATAGAAAACTCTTACGCATAGTTTACATTAAAATCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCttt                                                           <  1:3752197/100‑1 (MQ=255)                       cccACTTATAGAAAACTCTTACGCATAGTTTACATTAAAATCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAatcaatca                                     >  1:4447731/1‑100 (MQ=255)                       cccACTTATAGAAAACTCTTACGCATAGTTTACATTAAAATCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAatcaatca                                     >  1:4524526/1‑100 (MQ=255)                                          tACGCATAGTTTACATTAAAATCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAATCAATCATTCTTCATTAGTTTAAAGc                  >  1:4719082/1‑100 (MQ=255)                                              cATAGTTTACATTAAAATCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAATCAATCATTCTTCATTAGTTTAAAGCaaaa              <  1:894626/100‑1 (MQ=255)                                                          gtgaaTCAGACATTGAGGAATGA‑TTTTTAATTTCTTCAGCTTTATTGAAATTATAAAATCAATCATTCTTCATTAGTTTAAAGCAAAAAAATATTGatat  >  1:2400520/4‑100 (MQ=255)                                                                                 |                                                                              ATTTTTTCAACATTTGATCACTCCCACTTATAGAAAACTCTTACGCATAGTTTACATTAAAATCAGACATTGAGGAATGATTTTTTAATTTCTTCAGCTTTATTGAAATTCTAAAATCAATCATTCTTCATTAGTTTAAAGCAAAAAAATATTGATAT  >  NC_007793/973894‑974051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑