| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 901,719 | Δ2 bp | coding (439‑440/465 nt) | SAUSA300_RS04425 → | iron‑sulfur cluster assembly scaffold protein NifU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 901,719 | 0 | G | . | 88.1% | 114.0 / 14.7 | 43 | coding (439/465 nt) | SAUSA300_RS04425 | iron‑sulfur cluster assembly scaffold protein NifU |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base . (37/0); minor base T (5/0); total (43/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| * | NC_007793 | 901,720 | 0 | C | . | 86.1% | 125.2 / 20.3 | 43 | coding (440/465 nt) | SAUSA300_RS04425 | iron‑sulfur cluster assembly scaffold protein NifU |
| Reads supporting (aligned to +/- strand): ref base C (6/0); new base . (37/0); total (43/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
GAAGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAAAGCAGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATATTAGAC > NC_007793/901620‑901780 || taaGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAg > 1:833951/2‑100 (MQ=255)caaGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAg > 1:2981130/2‑100 (MQ=255) aaGCATTGCAAGGTGTATCTCAATTCCTAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAga > 1:4319455/1‑99 (MQ=255) aaGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGATACAga > 1:3611602/1‑99 (MQ=255) gCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatc > 1:1123719/1‑97 (MQ=255) cttGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAGGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcgg > 1:709044/2‑95 (MQ=255) aTTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAGGGTACAgatcgg > 1:1585831/1‑95 (MQ=255) ttGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGATGGTACAgatcgga > 1:984275/1‑94 (MQ=255) ttGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAGATCGGAAg > 1:4565587/1‑100 (MQ=255) ttGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAGATCGGAAg > 1:4331167/1‑100 (MQ=255) ttGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcgga > 1:1063501/1‑94 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAGATCGGAAga > 1:1068379/1‑99 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:4729983/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:212787/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:1511168/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:16628/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:2715010/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTACAgatcggaa > 1:5631136/1‑93 (MQ=255) tGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGATAGATCGGAAga > 1:400642/1‑99 (MQ=255) gCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAGATCGGAAgag > 1:4720101/1‑98 (MQ=255) tccAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAAAGCAGAAGGTACGACTGAAGAagaatag > 1:4669177/2‑100 (MQ=255) tgtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGat > 1:2094691/1‑100 (MQ=255) tgtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGat > 1:125652/1‑100 (MQ=255) tgtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGat > 1:2347255/1‑100 (MQ=255) tgtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGGACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGat > 1:3943617/1‑100 (MQ=255) ttgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:3193087/2‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGTAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:4593809/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:2224275/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:2533951/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:3829714/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:4115808/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:5336184/1‑100 (MQ=255) gtgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTCGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:5198238/1‑100 (MQ=255) ctgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:5063897/2‑100 (MQ=255) ctgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:3422969/2‑100 (MQ=255) atgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGata > 1:3856274/2‑100 (MQ=255) tgCCACATTAGCTTGGGAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGatat > 1:4732189/1‑100 (MQ=255) tgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGatat > 1:3885281/1‑100 (MQ=255) tgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGatat > 1:3874719/1‑100 (MQ=255) tgCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGatat > 1:2586311/1‑100 (MQ=255) agCCACATTAGCTTGGAAAACATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGatat > 1:3640132/2‑100 (MQ=255) tccACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:1777770/2‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:676206/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:1001270/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:841473/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:124446/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:545856/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:4232835/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:3364098/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:1834256/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:2798754/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:2726282/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:2523474/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:2227677/1‑100 (MQ=255) gCCACATTAGCTTGGAAAGCATTGGAAAAAGGCACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:4725057/1‑100 (MQ=255) atcacaTTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATAtt > 1:2119662/3‑100 (MQ=255) ccACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATATTa > 1:4689947/1‑100 (MQ=255) caTTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑AGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATATTAGAc > 1:387754/1‑100 (MQ=255) || GAAGCATTGCAAGGTGTATCTCAATTCCCAGCTCGTATTAAATGTGCCACATTAGCTTGGAAAGCATTGGAAAAAGGTACTGTTGCTAAAGAAGGTAAAGCAGAAGGTACGACTGAAGAAGAATAGAATGCTGTTAATCATAGATAATTTTGATATTAGAC > NC_007793/901620‑901780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |