Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	856,210	(T)8→7	intergenic (‑34/+158)	SAUSA300_RS04140 ← / ← SAUSA300_RS04145	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	856,203	0	T	.	100.0%	80.5 / NA	22	intergenic (‑27/+165)	SAUSA300_RS04140/SAUSA300_RS04145	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/20);  total (2/20)

TGACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATCTTTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTTATAGTA  >  NC_007793/856124‑856289                                                                                |                                                                                       tGACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCa                                                                   >  1:351355/1‑100 (MQ=255)   tctttGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACg                                                                 <  1:4682971/97‑1 (MQ=255)   atcTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCAct                                                                 <  1:3813282/98‑2 (MQ=255)     cTTGTACGTTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACggg                                                               <  1:1565812/100‑1 (MQ=255)        gTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGAc                                                            <  1:5245384/100‑1 (MQ=255)         tACATTTCCCCATGTAGCCTCTTCTAGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACt                                                           <  1:4379977/100‑1 (MQ=255)          aCATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACgggggcag                                                          <  1:1897331/100‑5 (MQ=255)          aCATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTg                                                          <  1:940258/100‑1 (MQ=255)                  tctcttccgatcttctTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCttt                                                  <  1:1198830/90‑1 (MQ=255)                  ccATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCttt                                                  <  1:1008764/100‑1 (MQ=255)                   cATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCttat                                                 <  1:81841/100‑3 (MQ=255)                          ccTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCGCGGGGACTGATTTCTTTAACAccgt                                          <  1:186651/100‑3 (MQ=255)                          ccTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCag                                          <  1:4363302/100‑2 (MQ=255)                           ctctTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCagg                                         <  1:3230002/100‑3 (MQ=255)                           ccgatcttGTTTCAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCagg                                         <  1:2376047/96‑3 (MQ=255)                                 ttGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAacac                                   <  1:2902571/100‑1 (MQ=255)                                  tGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAAcaca                                  >  1:4980775/1‑100 (MQ=255)                                   gTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAg                                 <  1:3354829/100‑1 (MQ=255)                                    ttttAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGGCTGATTTCTTTAACACCATTAAAcacaat                                <  1:5505455/100‑3 (MQ=255)                                                      tGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCAt              <  1:1808636/100‑1 (MQ=255)                                                              ttACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTtat      <  1:3520099/100‑1 (MQ=255)                                                                  aaaTTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTTATAGTa  <  1:4567753/100‑1 (MQ=255)                                                                                |                                                                                       TGACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATCTTTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTTATAGTA  >  NC_007793/856124‑856289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑