Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	838,047	(T)7→6	intergenic (+45/+271)	SAUSA300_RS04050 → / ← SAUSA300_RS04055	sporulation regulator WhiA/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	838,041	0	T	.	100.0%	49.3 / NA	14	intergenic (+39/+277)	SAUSA300_RS04050/SAUSA300_RS04055	sporulation regulator WhiA/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/0);  total (14/0)

TTTAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACACTTTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTGGAAAGTTTAATGAAGGAAATTC  >  NC_007793/837960‑838122                                                                                  |                                                                                  tttAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAgt                                                                >  1:1032773/1‑100 (MQ=255)  ttAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAgtg                                                               >  1:1118693/1‑100 (MQ=255)   tAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAgtgt                                                              >  1:4942793/1‑100 (MQ=255)          accccttAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGa                                                       >  1:2958741/6‑100 (MQ=255)           aaGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGaa                                                      >  1:908051/1‑100 (MQ=255)                        gTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGacta                                         >  1:1386129/1‑100 (MQ=255)                         tGAACAAATAGAATTATACGTAAGAAGGTGTTTTTGGTAGCGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGactaa                                        >  1:5490112/1‑100 (MQ=255)                          gAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGactaac                                       >  1:4870759/1‑100 (MQ=255)                            aCAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGacaaacag                                     >  1:2550789/1‑98 (MQ=255)                                          aagtaagAAGGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTgg                       >  1:2726122/1‑100 (MQ=255)                                             acagaagGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTGGgaa                    >  1:5116628/3‑100 (MQ=255)                                              aagaagGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTGGGAAg                   >  1:3951451/1‑100 (MQ=255)                                                 aagGTGTTTTTGGTAGTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGcagatcggaag                >  1:3675639/1‑90 (MQ=255)                                                               ggTGAATTATCAAAAACAC‑TTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTGGGAAGTTTAATGAAGGAAATTc  >  1:5215327/2‑100 (MQ=255)                                                                                  |                                                                                  TTTAGCCGATAAGATTAGAAATGGTGAACAAATAGAATTATAAGTAAGAAGGTGTTTTTGGTAGTGAATTATCAAAAACACTTTTTTTATGATTAAAAAGTGTTTAATGAATAATAGTCGACTAACTATTAAATTGAGCTGGAAAGTTTAATGAAGGAAATTC  >  NC_007793/837960‑838122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑