Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,803,422	T→C	T50A (ACA→GCA)	SAUSA300_RS14365 ←	accessory Sec system glycosyltransferase GtfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,803,422	0	T	C	100.0%	50.4 / NA	20	T50A (ACA→GCA)	SAUSA300_RS14365	accessory Sec system glycosyltransferase GtfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/19);  total (1/19)

TAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGTCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACGGTAAGCTTGT  >  NC_007793/2803327‑2803510                                                                                                |                                                                                         tAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGt                                                                                      <  1:63793/100‑1 (MQ=255) tAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGt                                                                                      <  1:1856538/100‑1 (MQ=255) tAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGt                                                                                      <  1:5999496/100‑1 (MQ=255) tAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGt                                                                                      <  1:4851329/100‑1 (MQ=255) caaCGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTga                                                                                      <  1:1191547/99‑2 (MQ=255)  aaCGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTa                                                                                     <  1:4798543/100‑1 (MQ=255)  aaCGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTa                                                                                     <  1:4529518/100‑1 (MQ=255)  aaCGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTACTTGATCATTAAACCCAATGTTTTGCGTTAATGCCTGTa                                                                                     <  1:1024747/100‑1 (MQ=255)   aCGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTAt                                                                                    <  1:3762479/100‑1 (MQ=255)             tgttgGTGCTAAAGAAATATCGCCAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTATGTTCTCTGag                                                                          <  1:4808502/100‑2 (MQ=255)                tgGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGt                                                                       <  1:4665668/100‑1 (MQ=255)                                                aCAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTg                                       <  1:2954788/100‑1 (MQ=255)                                                                            cccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACgg           <  1:3677141/100‑1 (MQ=255)                                                                            cccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACgg           <  1:5499852/100‑1 (MQ=255)                                                                            cccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACgg           <  1:5827683/100‑1 (MQ=255)                                                                            accAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACgg           >  1:5565970/2‑100 (MQ=255)                                                                             ccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACgag          <  1:4341656/100‑3 (MQ=255)                                                                             ccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACGGt          <  1:1845381/100‑1 (MQ=255)                                                                             ccAATGTTTTGCGTTAATGCCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACGGt          <  1:852623/100‑1 (MQ=255)                                                                                     ttGCGTTAATGCCTGTATATTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACGGTAAGCTtgg  <  1:4834518/100‑2 (MQ=255)                                                                                                |                                                                                         TAACGTGTACGTTGTTGGTGCTAAAGAAATATCACTAAAATACTGATACAACCAAATAACTTCTTGATCTTTAAACCCAATGTTTTGCGTTAATGTCTGTATGTTCTCTGACTGTATAAAATCTAAAAACACAAATTTAGTGTCTTGATTTGTACGTCTCAATAATTTAGCACGGTAAGCTTGT  >  NC_007793/2803327‑2803510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑