Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,685,939	T→G	S249A (TCT→GCT)	SAUSA300_RS13805 →	ATP‑dependent Clp protease ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,685,939	0	T	G	100.0%	81.8 / NA	29	S249A (TCT→GCT)	SAUSA300_RS13805	ATP‑dependent Clp protease ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/0);  total (29/0)

GATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAA  >  NC_007793/2685849‑2686004                                                                                           |                                                                  gATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTaa                                                          >  1:2168046/1‑100 (MQ=255)           aaCCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAtt                                                >  1:3884668/1‑100 (MQ=255)             ccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAtcgg                                              >  1:810229/1‑97 (MQ=255)             ccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                              >  1:4851588/1‑100 (MQ=255)             ccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                              >  1:1985039/1‑100 (MQ=255)             ccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                              >  1:3751923/1‑100 (MQ=255)             catGCATCAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAtcgg                                              >  1:2961832/3‑97 (MQ=255)              cTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTaa                                             >  1:2759075/1‑100 (MQ=255)               tGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACACGCAGAtcggaa                                            >  1:5617069/1‑95 (MQ=255)                tcATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                           >  1:2726070/2‑100 (MQ=255)                gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                           >  1:926317/1‑100 (MQ=255)                gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                           >  1:3319587/1‑100 (MQ=255)                       tccGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                    >  1:1224142/3‑100 (MQ=255)                       atCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                    >  1:973144/2‑100 (MQ=255)                       accGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                    >  1:2556132/3‑100 (MQ=255)                            ggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTa                               >  1:5984943/1‑100 (MQ=255)                            ggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTa                               >  1:1191740/1‑100 (MQ=255)                                                 gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAGTGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa          >  1:3750907/1‑100 (MQ=255)                                                 gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa          >  1:3100677/1‑100 (MQ=255)                                                 gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa          >  1:2017534/1‑100 (MQ=255)                                                 gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa          >  1:494285/1‑100 (MQ=255)                                                 gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa          >  1:1605328/1‑100 (MQ=255)                                                    acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg       >  1:2016458/1‑100 (MQ=255)                                                    acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg       >  1:4140507/1‑100 (MQ=255)                                                    acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg       >  1:72673/1‑100 (MQ=255)                                                      aaccCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGtt     >  1:5831558/1‑100 (MQ=255)                                                       acacAAGATGAATATCGAAACAATATTCTTAAAGATGATGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTg    >  1:1830159/1‑100 (MQ=255)                                                        cacaAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGa   >  1:1828425/1‑100 (MQ=255)                                                         ccaAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGaa  >  1:1961872/2‑100 (MQ=255)                                                                                           |                                                                  GATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAA  >  NC_007793/2685849‑2686004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑