Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,664,310	C→G	V61V (GTC→GTG)	SAUSA300_RS13685 →	N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,664,310	0	C	G	100.0%	24.9 / NA	11	V61V (GTC→GTG)	SAUSA300_RS13685	N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (5/6);  total (5/6)

AACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGG  >  NC_007793/2664219‑2664402                                                                                            |                                                                                             aaCCACAAACCATAGACGAACGTGCCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGtt                                                                                      <  1:24799/100‑1 (MQ=255)                               ggTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGcc                                                        <  1:5353326/100‑1 (MQ=255)                                gTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCa                                                       <  1:5529797/100‑1 (MQ=255)                                   tGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCa                                                    >  1:3982445/1‑100 (MQ=255)                                                 ctAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAAt                                      >  1:2790294/2‑100 (MQ=255)                                                   taGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCg                                    >  1:5017522/2‑100 (MQ=255)                                                                   ttGTATTTGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGt                    >  1:1382257/1‑100 (MQ=255)                                                                        catGAGGAAAATGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATg               >  1:369778/3‑100 (MQ=255)                                                                                aaaTGGAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCt       <  1:3845982/100‑1 (MQ=255)                                                                                    ggAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTaggt   <  1:4081129/100‑3 (MQ=255)                                                                                     gAAGTGTGTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAgg  <  1:171693/100‑1 (MQ=255)                                                                                            |                                                                                             AACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGG  >  NC_007793/2664219‑2664402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑