Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,651,710	G→A	A143T (GCA→ACA)	SAUSA300_RS13615 →	fructose 1,6‑bisphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,651,710	0	G	A	100.0%	11.4 / NA	6	A143T (GCA→ACA)	SAUSA300_RS13615	fructose 1,6‑bisphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/1);  total (5/1)

CTTAATGTCTGGTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAGCATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAATGAATATCAAAATAAAAAATCATATTACGAAACACTTGT  >  NC_007793/2651620‑2651801                                                                                           |                                                                                            cTTAATGTCTGGTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAACATTGCCat                                                                                    <  1:596651/100‑2 (MQ=255)           ggTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAACATTGCCAAAACAATACGt                                                                          >  1:3137082/1‑100 (MQ=255)           ggTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAACATTGCCAAAACAATACGt                                                                          >  1:3625725/1‑100 (MQ=255)           ggTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAACATTGCCAAAACAATACGt                                                                          >  1:4773732/1‑100 (MQ=255)           ggTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAACATTGCCAAAACAATACGt                                                                          >  1:5911639/1‑100 (MQ=255)                                                                                   ggCGTAAAACATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAATGAATATCAAAATAAAAAATCATATTACGAAACACTTGt  >  1:3955095/2‑100 (MQ=255)                                                                                           |                                                                                            CTTAATGTCTGGTATATCACAACAATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTGCGTAAAGCATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAATGAATATCAAAATAAAAAATCATATTACGAAACACTTGT  >  NC_007793/2651620‑2651801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑