Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,590,246	G→A	A14T (GCT→ACT)	SAUSA300_RS13320 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,590,246	0	G	A	100.0%	63.0 / NA	22	A14T (GCT→ACT)	SAUSA300_RS13320	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/4);  total (18/4)

TGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTT  >  NC_007793/2590163‑2590345                                                                                    |                                                                                                    tgtgCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACACTACCGAACAATcgcg                                                                                     <  1:1488938/100‑1 (MQ=255)                                     ggTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTa                                                 >  1:4576349/1‑100 (MQ=255)                                                 caaatacaaataAACATTACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGt                                     >  1:5925476/1‑100 (MQ=255)                                                             aaCATTACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGct                         <  1:1736794/100‑2 (MQ=255)                                                              aCATTACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCg                        <  1:2882472/100‑1 (MQ=255)                                                              aCATTACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCg                        <  1:3528656/100‑1 (MQ=255)                                                                  tACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCa                    >  1:796734/1‑100 (MQ=255)                                                                  tACATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCa                    >  1:2223476/1‑100 (MQ=255)                                                                   aCATAGAAGAAGAATACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCAt                   >  1:997343/1‑100 (MQ=255)                                                                             agcaTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttt         >  1:701781/4‑100 (MQ=255)                                                                              gtgtACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCtttt        >  1:1548974/4‑100 (MQ=255)                                                                               taTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:513493/2‑100 (MQ=255)                                                                               gaTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:4694380/2‑100 (MQ=255)                                                                               caTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:3326544/2‑100 (MQ=255)                                                                               caTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:3893667/2‑100 (MQ=255)                                                                               caTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:966305/2‑100 (MQ=255)                                                                               aaTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCttttt       >  1:4042044/1‑100 (MQ=255)                                                                                aTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTa      >  1:3478017/1‑100 (MQ=255)                                                                                aTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTa      >  1:4201816/1‑100 (MQ=255)                                                                                aTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTa      >  1:3317288/1‑100 (MQ=255)                                                                                aTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTa      >  1:5192513/1‑100 (MQ=255)                                                                                aTACACTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTa      >  1:2602930/1‑100 (MQ=255)                                                                                    gCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTGACATCTTGCCGATCATTATCGTCTTTTTAACtt  >  1:1346478/1‑100 (MQ=255)                                                                                    |                                                                                                    TGTGCTAAAGTATCTATATAATACAACTATTTAAGAGGTATACTATGTCAAATACAAATAAACATTACATAGAAGAAGAATACGCTACCGAACAATCGCGTTTTTTCAAACGTGATATTGGATTTATTTTCCTTTACATATTTTTGGTTAACATCTTGCCGATCATTATCGTCTTTTTAACTT  >  NC_007793/2590163‑2590345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑