Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,132,065	G→A	C257Y (TGT→TAT)	SAUSA300_RS10855 →	succinyl‑diaminopimelate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,132,065	0	G	A	100.0%	16.6 / NA	7	C257Y (TGT→TAT)	SAUSA300_RS10855	succinyl‑diaminopimelate desuccinylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

TTGCGCCTATGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATGTTCGATTATAAATGGCGGCAAACAATTTAACTCTGTACCAGATGAAGCTTCACTTG  >  NC_007793/2131984‑2132121                                                                                  |                                                         ctGCGCCTATGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATggcgg                                        >  1:3446018/2‑100 (MQ=255)   gcgcCTATGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCa                                      <  1:5598323/100‑1 (MQ=255)   gcgcCTATGTTCAAATCATTGATTGGAAAAGAAATTTCTAAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCa                                      <  1:2895943/100‑1 (MQ=255)      ccTATGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCAAAc                                   <  1:1010727/100‑1 (MQ=255)         aTGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCAAACAAt                                >  1:3506598/1‑100 (MQ=255)                      tgattgGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCAAACAATTTAACTCTGTAcc                   <  1:2448745/100‑1 (MQ=255)                                       tCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATATTCGATTATAAATGGCGGCAAACAATTTAACTCTGTACCAGATGAAGCTTCACTTg  <  1:6013521/100‑1 (MQ=255)                                                                                  |                                                         TTGCGCCTATGTTCAAATCATTGATTGGAAAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATGTTCGATTATAAATGGCGGCAAACAATTTAACTCTGTACCAGATGAAGCTTCACTTG  >  NC_007793/2131984‑2132121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑