Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007790 875 T→C V199A (GTC→GCC)  SAUSA300_RS14705 → replication protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077908750TC99.5% 574.5 / ‑5.3 206V199A (GTC→GCC) SAUSA300_RS14705replication protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (191/13);  total (193/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 209 total aligned reads displayed.

ATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAAG  >  NC_007790/778‑972
                                                                                                 |                                                                                                  
gttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCg                                                                                                  >  1:843477/2‑100 (MQ=255)
aTTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCg                                                                                                  <  1:1006902/100‑1 (MQ=255)
 ttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGa                                                                                                 <  1:5011852/100‑1 (MQ=255)
 ttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGa                                                                                                 >  1:640569/1‑100 (MQ=255)
 ttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGACGCCGa                                                                                                 <  1:5543929/100‑1 (MQ=255)
  tGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGaa                                                                                                >  1:3383167/1‑100 (MQ=255)
   gTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAg                                                                                               >  1:3021059/1‑100 (MQ=255)
    tAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGa                                                                                              >  1:2490995/1‑100 (MQ=255)
    caaGTGATTTAGAAAAGGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGa                                                                                              >  1:4580941/2‑100 (MQ=37)
     aaGTGATTTAGAAAAGGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:4777056/1‑100 (MQ=38)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:4820255/1‑100 (MQ=255)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:5275959/1‑100 (MQ=255)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:2026473/1‑100 (MQ=255)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:5562047/1‑100 (MQ=255)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:1248142/1‑100 (MQ=255)
     aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                                             >  1:2864288/1‑100 (MQ=255)
        cctcctAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGt                                                                                          >  1:3685594/6‑100 (MQ=37)
        atggttAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGt                                                                                          >  1:2759385/5‑100 (MQ=37)
         gATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTa                                                                                         >  1:3484889/1‑100 (MQ=255)
          tgttAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTaa                                                                                        >  1:2527639/3‑100 (MQ=255)
           cctAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAt                                                                                       >  1:1911072/3‑100 (MQ=255)
            ctAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAtt                                                                                      >  1:1209841/2‑100 (MQ=255)
            ccaGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAtt                                                                                      >  1:1573289/3‑100 (MQ=255)
            atAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAtcggaaga                                                                                      >  1:5899779/2‑93 (MQ=37)
             tAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAttt                                                                                     >  1:4902463/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTg                                                                                    >  1:3501277/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTg                                                                                    >  1:3888273/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTg                                                                                    >  1:4467787/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTg                                                                                    >  1:6030717/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTg                                                                                    >  1:1742007/1‑100 (MQ=255)
              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGACGCCGAAGAtcggaagagc                                                                                    >  1:5784361/1‑91 (MQ=25)
                aaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTggg                                                                                  <  1:2741165/100‑3 (MQ=255)
                  aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                                                                >  1:58697/1‑100 (MQ=255)
                  aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                                                                >  1:2932726/1‑100 (MQ=255)
                    taccttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAAt                                                                              >  1:1193439/5‑100 (MQ=255)
                       tttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTGATACa                                                                           <  1:1028637/100‑1 (MQ=37)
                       tttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                                           >  1:1423405/1‑100 (MQ=255)
                       ttggTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                                           >  1:2392155/4‑100 (MQ=38)
                       actGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                                           >  1:2654868/3‑100 (MQ=38)
                        ttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACag                                                                          <  1:4057776/100‑2 (MQ=38)
                        ttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACaa                                                                          >  1:5966029/1‑100 (MQ=255)
                        ttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACaa                                                                          >  1:1466927/1‑100 (MQ=255)
                          gTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGt                                                                        >  1:1440997/1‑100 (MQ=255)
                            aTCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtga                                                                      >  1:5758275/1‑100 (MQ=255)
                              cGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtgatg                                                                    >  1:5159370/1‑100 (MQ=39)
                              cGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGggatg                                                                    >  1:796853/1‑100 (MQ=38)
                               gAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGagatga                                                                   <  1:5989679/100‑1 (MQ=38)
                                ctgccGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGaa                                                                  >  1:518883/5‑100 (MQ=255)
                                   aCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                               >  1:136129/1‑100 (MQ=255)
                                   aCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                               >  1:2098746/1‑100 (MQ=255)
                                   aCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                               >  1:346688/1‑100 (MQ=255)
                                   aCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                               >  1:4725517/1‑100 (MQ=255)
                                   aCGTATGTCAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                               >  1:357932/1‑100 (MQ=255)
                                    atgtggTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATa                                                              >  1:3825892/6‑100 (MQ=255)
                                      tATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaa                                                            >  1:1819171/1‑100 (MQ=255)
                                       gggTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaaa                                                           >  1:222478/3‑100 (MQ=255)
                                       aTGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaga                                                           >  1:3170319/1‑98 (MQ=255)
                                           tAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gac                                                      >  1:2686702/1‑100 (MQ=255)
                                            aaGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacgaaa                                                      <  1:4451518/100‑5 (MQ=255)
                                              cttATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGa                                                   >  1:705894/2‑100 (MQ=255)
                                               gtATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaaaagacaaat                                                   >  1:1408482/2‑92 (MQ=255)
                                               gtATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑AACAGat                                                  >  1:1300256/2‑99 (MQ=255)
                                                tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gaccagat                                                 >  1:201705/1‑95 (MQ=255)
                                                tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAGTC‑GACAGatc                                                 >  1:4410196/1‑98 (MQ=255)
                                                tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAGAC‑GACAGatc                                                 >  1:3103065/1‑98 (MQ=255)
                                                tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GGCAGatc                                                 >  1:3817255/1‑98 (MQ=255)
                                                tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑CATAGatc                                                 >  1:63009/1‑98 (MQ=255)
                                                caTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGAAATTTGATTAATACAAGTGATGAAGATAAAGC‑GACAGatc                                                 >  1:5404139/2‑98 (MQ=255)
                                                aaTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaaagagacagat                                                  >  1:215520/2‑91 (MQ=255)
                                                 ttggtggATTGCTTAAACAAAAGCATAAAATTTTGAACTTAGACGATGCCGAAGCTGGTAATTTGATTAATACAAGTGATGAAGATAAAACGAACAGatc                                                 >  1:3945596/2‑98 (MQ=255)
                                                 ctggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑aacgagatc                                                >  1:1094588/2‑94 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGCTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAGC‑GACAGatcg                                                >  1:2143505/1‑97 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacccagatcgg                                                 >  1:727524/1‑91 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gactagatc                                                >  1:1747073/1‑94 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gacgagatc                                                >  1:3997144/1‑94 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAGC‑GACAGatcg                                                >  1:1800831/1‑97 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGatcg                                                >  1:4535430/1‑97 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatcg                                                >  1:5511743/1‑97 (MQ=255)
                                                 aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACga                                                >  1:733454/1‑100 (MQ=255)
                                                  atgtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAATC‑GACAGatcgg                                               >  1:4365513/3‑96 (MQ=255)
                                                   ggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatcgga                                              >  1:2698508/1‑95 (MQ=255)
                                                   ggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACgaag                                              >  1:4031510/1‑100 (MQ=255)
                                                   cgtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGatcgga                                              >  1:2894451/2‑95 (MQ=255)
                                                   aggggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGatcgga                                              >  1:507667/4‑95 (MQ=255)
                                                            tatAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGc                                    >  1:4213034/3‑100 (MQ=255)
                                                               aaaCAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGcaca                                 >  1:5726049/1‑100 (MQ=255)
                                                                    aaagCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCaa                            >  1:5459209/1‑100 (MQ=255)
                                                                    aaagCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCaa                            >  1:2335006/1‑100 (MQ=255)
                                                                                       ctAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGGAAtag         >  1:676444/2‑98 (MQ=255)
                                                                                         aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAAttggaagatcgg       >  1:30534/1‑90 (MQ=255)
                                                                                         aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGGaaagatc       >  1:1959232/1‑95 (MQ=255)
                                                                                         aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATATGGaagatcg       >  1:1678343/1‑95 (MQ=255)
                                                                                         aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAACTTGGaagatcg       >  1:5286488/1‑95 (MQ=255)
                                                                                           atgATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGaaagatcgga     >  1:4799340/3‑93 (MQ=255)
                                                                                           aCGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAAGTTGGaagatcgga     >  1:867743/1‑93 (MQ=255)
                                                                                            cGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGGaagatcggaa    >  1:3780766/1‑92 (MQ=255)
                                                                                             gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGGaagatcggaag   >  1:204461/1‑91 (MQ=255)
                                                                                             gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAAGTCGGaagatcggaag   >  1:2995163/1‑91 (MQ=255)
                                                                                              aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAATTTGGaagatcggaaga  >  1:4616911/1‑90 (MQ=255)
                                                                                              aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCAATTACCGCAAGTTGGaagatcggaaga  >  1:3266017/1‑90 (MQ=255)
                                                                                                 |                                                                                                  
ATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAAG  >  NC_007790/778‑972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑