Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,961,354	C→T	noncoding (9/74 nt)	SAUSA300_RS09735 ←	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,961,354	0	C	T	100.0%	185.2 / NA	61	noncoding (9/74 nt)	SAUSA300_RS09735	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/58);  total (3/58)

ACGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAGAACTTTTTG  >  NC_007793/1961260‑1961428                                                                                               |                                                                           aCGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccc                                                                       <  1:3842065/100‑1 (MQ=255)  tgagaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATcccc                                                                      <  1:2127346/99‑2 (MQ=255)    tctccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:5569991/97‑1 (MQ=255)    agaCCTATTAAATTAAAGACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:4249208/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:1019869/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    >  1:790152/1‑100 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:6516/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:4710916/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:3142819/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:5207506/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:2172688/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                    <  1:5302498/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATACcgcg                                                                    <  1:1467159/100‑1 (MQ=255)     gaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcga                                                                   <  1:1480868/100‑2 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcgta                                                                  <  1:4401162/100‑3 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcggg                                                                  <  1:1574467/100‑3 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcctg                                                                  <  1:4576643/100‑4 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATcccccactc                                                                 <  1:1148492/100‑7 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                 <  1:2271348/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                 <  1:4891767/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                 <  1:2699863/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                 <  1:3202839/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                 <  1:3218855/100‑1 (MQ=255)       ccTATTAAATT‑AAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTag                                                                <  1:4848575/100‑2 (MQ=255)          attaaattaaAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAatg                                                              <  1:4740382/100‑2 (MQ=255)             aaattaaaAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAAtatta                                                           <  1:4370443/100‑1 (MQ=255)              aattaaaAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAAtattat                                                          <  1:4299707/100‑1 (MQ=255)                   aaaaCTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGa                                                     <  1:4059833/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                <  1:5409019/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                <  1:1329994/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                <  1:4437151/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                <  1:5428518/100‑1 (MQ=255)                         aTGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAca                                               <  1:2961154/100‑2 (MQ=255)                          tGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCt                                              <  1:1833245/100‑1 (MQ=255)                          tGTATTGCGGAAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAcgg                                              <  1:6023502/100‑3 (MQ=255)                           gTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAGTTAccac                                             <  1:4790895/100‑3 (MQ=255)                           gTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAccag                                             <  1:3169347/100‑3 (MQ=255)                           gTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAccac                                             <  1:108864/100‑3 (MQ=255)                           gTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAccac                                             <  1:1468377/100‑3 (MQ=255)                              ttGCGGGAGGCGGATTTGGACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAcccctgt                                          <  1:4710452/100‑6 (MQ=17)                                      ggCGGATTTGAACCACCGCCCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATAcc                                  <  1:5163432/100‑1 (MQ=255)                                      ggCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATAcc                                  <  1:1120348/100‑1 (MQ=255)                                         ggATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCAtt                               <  1:4550793/100‑2 (MQ=255)                                          gATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATag                              <  1:2876848/100‑2 (MQ=255)                                          gATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATac                              <  1:3540002/100‑2 (MQ=255)                                                aaCCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCaagt                        <  1:3670822/100‑3 (MQ=255)                                                     ccGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTa                   <  1:655034/100‑1 (MQ=255)                                                      cGACCTTCGGTTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTac                  <  1:3174487/100‑2 (MQ=255)                                                      cGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATTCCATAATCAAAAACCTaa                  <  1:1474211/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTGCCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:25205/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:2439123/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:5079836/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:2417316/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:2159371/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:1608019/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:3210552/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTACCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa             <  1:4892842/100‑1 (MQ=255)                                                            ttgggTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCaa            >  1:1517805/3‑100 (MQ=255)                                                             cGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAg           <  1:506975/100‑1 (MQ=255)                                                              gggCTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCCAAAGTCAAGa          <  1:1703155/100‑1 (MQ=255)                                                                      aGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAGAACttgggc  >  1:4431599/1‑96 (MQ=255)                                                                                               |                                                                           ACGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAGAACTTTTTG  >  NC_007793/1961260‑1961428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑